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Human DC-SIGNR/CD299 Antibody

Catalog #: MAB162
18 Citations Datasheet / COA / SDS
Clone 120604 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB162
MAB162-SP
Detection of DC‑SIGNR/CD299 in Human DC‑SIGNR/CD299 Transfected 3T3 Mouse Cell Line by Flow Cytometry.
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Citations (18)
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Human DC-SIGNR/CD299 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DC‑SIGNR/CD299.
Source
Monoclonal Mouse IgG2B Clone # 120604
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGNR/CD299
Accession # Q9H2X3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
Adhesion Blockade
The adhesion of NIH-3T3 mouse embryonic fibroblast cells (5 x 104 cells/well) to immobilized Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC, 5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 2.5 µg/mL of the antibody.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of DC-SIGNR/CD299 antibody in Human DC-SIGNR/CD299 Transfected 3T3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of DC‑SIGNR/CD299 in Human DC‑SIGNR/CD299 Transfected 3T3 Mouse Cell Line by Flow Cytometry. Human DC-SIGNR/CD299 and DC-SIGN transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC-SIGNR/CD299 Monoclonal Antibody (Catalog # MAB162, filled histograms) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Immunocytochemistry/ Immunofluorescence Detection of Human DC-SIGNR/CD299/CLEC4M by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human DC-SIGNR/CD299/CLEC4M by Immunocytochemistry/Immunofluorescence In situ localization of Ptx3-LECs and transition between Ptx3-LECs and Marco-LECs in human LNs. (A) Expression of CD36/Cd36 in LN LEC subsets of human and mouse. Dots indicate mean log-normalized transcript count. (B–D) Identification of CD36high Ptx3-LECs in human head and neck LNs by immunostaining. (B,C) Immunofluorescence of PROX-1, MARCO and CD36 (B), or PROX-1, LYVE-1 and CLEC4M (C). Zoomed-in images (inset marked by blue dotted lines) in (B) and (C) demonstrate CD36high LYVE-1+ paracortical sinuses (filled arrowhead). Scale bars = 500 μm (left panels) and 100 μm (right panel inset). (D) CD36high LYVE-1+ Ptx3-LECs (filled arrowhead) can be seen associated with MARCO+ CLEC4M+ Marco-LECs (empty arrowhead) in human LNs. Scale bars = 100 μm. CD36high Ptx3-LECs were detected in four out of seven human LNs. Images are representative of four biological replicates. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32426372), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human DC-SIGNR/CD299/CLEC4M by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human DC-SIGNR/CD299/CLEC4M by Immunocytochemistry/Immunofluorescence In situ localization of Ptx3-LECs and transition between Ptx3-LECs and Marco-LECs in human LNs. (A) Expression of CD36/Cd36 in LN LEC subsets of human and mouse. Dots indicate mean log-normalized transcript count. (B–D) Identification of CD36high Ptx3-LECs in human head and neck LNs by immunostaining. (B,C) Immunofluorescence of PROX-1, MARCO and CD36 (B), or PROX-1, LYVE-1 and CLEC4M (C). Zoomed-in images (inset marked by blue dotted lines) in (B) and (C) demonstrate CD36high LYVE-1+ paracortical sinuses (filled arrowhead). Scale bars = 500 μm (left panels) and 100 μm (right panel inset). (D) CD36high LYVE-1+ Ptx3-LECs (filled arrowhead) can be seen associated with MARCO+ CLEC4M+ Marco-LECs (empty arrowhead) in human LNs. Scale bars = 100 μm. CD36high Ptx3-LECs were detected in four out of seven human LNs. Images are representative of four biological replicates. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32426372), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DC-SIGNR/CD299

Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN or CD299) and DC-SIGN related protein (DC-SIGNR, DC-SIGN2, L-SIGN or CD209L) are type II membrane proteins that are mannose-specific calcium-dependent (C-type) lectins. The two proteins share 77% amino acid identity. DC-SIGN mediates interactions between dendritic cells (DCs) and T cells. Both DC-SIGN and DC-SIGNR have been shown to bind HIV, hepatitis C glycoproteins, Ebola virus glycoproteins and the cellular adhesion protein ICAM-3 (1‑4). DC-SIGN and DC-SIGNR appear to selectively recognize and bind viral proteins containing a large portion of high-mannose oligosaccharides (5). Though DC-SIGN and DC-SIGNR are found on the same chromosome, they are not expressed in the same tissue. DC-SIGN is expressed solely on Dendritic cells while DC-SIGNR is found on endothelial cells in the liver and lymph node sinuses and in a significant portion of capillary endothelial cells in term placenta (1, 4).

References
  1. Pohlmann, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670.
  2. Pohlmann, S. et al. (2003) J. Virol. 77:4070.
  3. Simmons, L.G. et al. (2003) J. Virol. 77:1337.
  4. Bahirova, A.A. et al. (2001) J. Exp. Med. 193:671.
  5. Feinberg, H. et al. (2001) Science 294:2163.
Long Name
Dendritic Cell-specific ICAM-3-grabbing Non-integrin 2
Entrez Gene IDs
10332 (Human)
Alternate Names
CD209L; CD209L1; CD209Ldendritic cell-specific ICAM-3-grabbing nonintegrin 2; CD299 antigenMGC129964; CD299; CLEC4M; C-type lectin domain family 4, member M; DC-SIGN2; DC-SIGN2CD209 antigen-like protein 1; DCSIGNR; DC-SIGNR; DC-SIGNRDC-SIGN-related protein; DCSIGNRMGC47866; Dendritic cell-specific ICAM-3-grabbing non-integrin 2; HP10347; liver/lymph node-specific ICAM-3 grabbing non-integrin; Liver/lymph node-specific ICAM-3-grabbing non-integrin; LSIGN; L-SIGN; LSIGNC-type lectin domain family 4 member M; mannose binding C-type lectin DC-SIGNR

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Citations for Human DC-SIGNR/CD299 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Envelope Protein Glycosylation Mediates Zika Virus Pathogenesis
    Authors: Derek L. Carbaugh, Ralph S. Baric, Helen M. Lazear
    Journal of Virology
  2. A Single-Cell Transcriptional Roadmap of the Mouse and Human Lymph Node Lymphatic Vasculature
    Authors: Menglan Xiang, Rubén Adrián Grosso, Akira Takeda, Junliang Pan, Tove Bekkhus, Kevin Brulois et al.
    Frontiers in Cardiovascular Medicine
  3. N-glycosylation of viral glycoprotein is a novel determinant for the tropism and virulence of highly pathogenic tick-borne bunyaviruses
    Authors: Shimojima, M;Sugimoto, S;Taniguchi, S;Maeki, T;Yoshikawa, T;Kurosu, T;Tajima, S;Lim, CK;Ebihara, H;
    PLoS pathogens
    Species: Human, Primate - Cercopithecus aethiops (African Green Monkey)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Human Monoclonal Antibodies against NS1 Protein Protect against Lethal West Nile Virus Infection
    Authors: AW Wessel, MP Doyle, TB Engdahl, J Rodriguez, JE Crowe, MS Diamond
    MBio, 2021-10-12;12(5):e0244021.
    Species: Human
    Sample Types: Whole Cells
  5. L-SIGN is a receptor on liver sinusoidal endothelial cells for SARS-CoV-2 virus
    Authors: Y Kondo, JL Larabee, L Gao, H Shi, B Shao, CM Hoover, JM McDaniel, YC Ho, R Silasi-Man, SA Archer-Har, P Azadi, RS Srinivasan, AR Rezaie, A Borczuk, JC Laurence, F Lupu, J Ahamed, RP McEver, JF Papin, Z Yu, L Xia
    JCI Insight, 2021-07-22;6(14):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  6. Dengue viruses infect human megakaryocytes, with probable clinical consequences
    Authors: MB Vogt, A Lahon, RP Arya, JL Spencer Cl, R Rico-Hesse
    PLoS Negl Trop Dis, 2019-11-25;13(11):e0007837.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. The endothelial cell receptor stabilin-2 regulates VWF-FVIII complex half-life and immunogenicity
    Authors: LL Swystun, JD Lai, C Notley, I Georgescu, AS Paine, J Mewburn, K Nesbitt, K Schledzews, C Géraud, J Kzhyshkows, S Goerdt, W Hopman, RR Montgomery, PD James, D Lillicrap
    J. Clin. Invest., 2018-08-20;128(9):4057-4073.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2.
    Authors: Johnson TR, McLellan JS, Graham BS
    J. Virol., 2011-11-16;86(3):1339-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. DC-SIGN mediates cell-free infection and transmission of human T-cell lymphotropic virus type 1 by dendritic cells.
    Authors: Jain P, Manuel SL, Khan ZK, Ahuja J, Quann K, Wigdahl B
    J. Virol., 2009-08-19;83(21):10908-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
    Authors: Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH
    J. Hepatol., 2009-07-30;51(4):734-49.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. DC-SIGN and CLEC-2 mediate human immunodeficiency virus type 1 capture by platelets.
    Authors: Chaipan C, Soilleux EJ, Simpson P, Hofmann H, Gramberg T, Marzi A, Geier M, Stewart EA, Eisemann J, Steinkasserer A, Suzuki-Inoue K, Fuller GL, Pearce AC, Watson SP, Hoxie JA, Baribaud F, Pohlmann S
    J. Virol., 2006-09-01;80(18):8951-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. L-SIGN (CD209L) isoforms differently mediate trans-infection of hepatoma cells by hepatitis C virus pseudoparticles.
    Authors: Falkowska E, Durso R, Gardner J, Cormier E, Arrigale R, Ogawa R, Donovan G, Maddon P, Olson W, Dragic T
    J Gen Virol, 2006-09-01;87(0):2571-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
    Authors: Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S
    Gut, 2006-07-13;56(2):243-52.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  14. Expression of DC-SIGN and DC-SIGNR on human sinusoidal endothelium: a role for capturing hepatitis C virus particles.
    Authors: Lai WK, Sun PJ, Zhang J, Jennings A, Lalor PF, Hubscher S, McKeating JA, Adams DH
    Am. J. Pathol., 2006-07-01;169(1):200-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  15. Hepatitis C virus targets DC-SIGN and L-SIGN to escape lysosomal degradation.
    Authors: Ludwig IS, Lekkerkerker AN, Depla E, Bosman F, Musters RJ, Depraetere S, van Kooyk Y, Geijtenbeek TB
    J. Virol., 2004-08-01;78(15):8322-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Epigenetic glycosylation of SARS-CoV-2 impact viral infection through DC&L-SIGN receptors
    Authors: Lei Guo, Yan Liang, Heng Li, Huiwen Zheng, Zening Yang, Yanli Chen et al.
    iScience
  17. A protective human monoclonal antibody targeting the West Nile virus E protein preferentially recognizes mature virions.
    Authors: Goo L, Debbink K, Kose N et al.
    Nat Microbiol.
  18. CLEC4M-positive and CD81-negative Huh7 cells are not susceptible to JFH-1 HCVcc infection but mediate transinfection
    Authors: Mariko Ishibashi, Naoko Morita, Chisato Nomura-Kawaguchi, Yohko Shimizu, Takaji Wakita, Mariko Esumi
    Archives of Virology

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