Human DDX17 Antibody

Catalog #: AF4530 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4530
AF4530-SP

Detection of Human DDX17 by Western Blot.

3 Images

All DDX17 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DDX17 Antibody Summary

Species Reactivity

Human

Specificity

Detects human DDX17 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human DDX17
Asn498-Tyr638
Accession # Q92841

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Immunocytochemistry

1-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human DDX17 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, HEK293 human embryonic kidney cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human DDX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4530) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DDX17 at approximately 86 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

View Larger

DDX17 in A431 Human Cell Line. DDX17 was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human DDX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4530) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

View Larger

DDX17 in Human Liver Cancer Tissue. DDX17 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Goat Anti-Human DDX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4530) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DDX17

DDX17 is a member of the DEAD box family of proteins, a family of RNA helicases characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). DDX17 plays an essential role in many aspects of cellular RNA metabolism, including RNA transport, transcription, ribosome assembly, spliceosome function, RNA degradation, and the initiation of translation. Distribution patterns suggests that some members of this family may be involved in spermatogenesis, embryogenesis, and cellular growth and division. Alternative splicing of the DDX17 gene generates two transcript variants encoding isoforms p72 and p82. p82 is translated from a non-AUG start codon 243 bp upstream of the first in frame AUG initiator codon for p72. DDX17 is more closely related to DDX5 than to any other member of the DEAD box family.

Long Name

DEAD Box Protein 17

Entrez Gene IDs

10521 (Human); 67040 (Mouse); 315133 (Rat)

Alternate Names

DDX17; DEAD (Asp-Glu-Ala-Asp) box polypeptide 17; DEAD box protein 17; DEAD box protein p72; DEAD/H box 17; DKFZp761H2016; EC 3.6.1; EC 3.6.4.13; p72; P72DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 17 (72kD); probable ATP-dependent RNA helicase DDX17; RH70; RNA-dependent helicase p72