Human DEC-205/CD205 Antibody

Name: Human DEC-205/CD205 Antibody
Brand: R&D Systems
SKU: MAB2047
Rating: 4 (1 reviews)

Catalog #: MAB2047 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2047
MAB2047-SP

Detection of DEC‑205/CD205 in Human Monocytes by Flow Cytometry.

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All DEC-205/CD205 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DEC-205/CD205 Antibody Summary

Species Reactivity

Human

Specificity

Detects human DEC‑205/CD205 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse DEC-205 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 523203

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human DEC‑205/CD205
Cys216-Cys501
Accession # O60449

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of DEC‑205/CD205 in Human Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human DEC-205/CD205 Monoclonal Antibody (Catalog # MAB2047, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DEC-205/CD205

DEC-205, also known as CD205 and lymphocyte antigen 75 (Ly 75), is a type I transmembrane protein that is primarily expressed on dendritic cells and thymic epithelial cells. The extracellular region of DEC-205 contains ten C-type lectin-like domains, a fibronectin type II domain and a ricin B-type lectin domain. DEC-205 functions as an endocytic receptor for antigens. The recombinant protein used to generate the anti-human DEC-205 antibody contains the first two C-type lectin domains.

Entrez Gene IDs

4065 (Human); 17076 (Mouse)

Alternate Names

CD205; CLEC13B; C-type lectin domain family 13 member B; DEC205; DEC-205; GP200-MR6; Ly75; Ly-75; Lymphocyte antigen 75