Human DEC-205/CD205 Fluorescein-conjugated Antibody

Name: Human DEC-205/CD205 Fluorescein-conjugated Antibody
Brand: R&D Systems
SKU: FAB2047F

Catalog #: FAB2047F Datasheet / COA / SDS

Discontinued Product

FAB2047F has been discontinued.

View all DEC-205/CD205 products.

Detection of DEC‑205/CD205 in Human Monocytes by Flow Cytometry.

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All DEC-205/CD205 Antibodies

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Human DEC-205/CD205 Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human DEC‑205/CD205 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse DEC-205 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 523203

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human DEC‑205/CD205
Cys216-Cys501
Accession # O60449

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of DEC‑205/CD205 in Human Monocytes by Flow Cytometry. Human monocytes were stained with Mouse Anti-Human DEC-205/CD205 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2047F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: DEC-205/CD205

DEC-205, also known as CD205 and lymphocyte antigen 75 (Ly 75), is a type I transmembrane protein that is primarily expressed on dendritic cells and thymic epithelial cells. The extracellular region of DEC-205 contains ten C-type lectin-like domains, a fibronectin type II domain and a ricin B-type lectin domain. DEC-205 functions as an endocytic receptor for antigens. The recombinant protein used to generate the anti-human DEC-205 antibody contains the first two C-type lectin domains.

Entrez Gene IDs

4065 (Human); 17076 (Mouse)

Alternate Names

CD205; CLEC13B; C-type lectin domain family 13 member B; DEC205; DEC-205; GP200-MR6; Ly75; Ly-75; Lymphocyte antigen 75