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Human Decorin Antibody

Catalog #: AF143
29 Citations Datasheet / COA / SDS
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AF143
AF143-SP
Detection of Human Decorin by Western Blot.
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Product Details
Citations (29)
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Human Decorin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Decorin in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Decorin
Gly17-Lys359
Accession # P07585
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunohistochemistry
10-20 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Decorin antibody by Western Blot. View Larger

Detection of Human Decorin by Western Blot. Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Decorin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Decorin antibody in Human Breast by Immunohistochemistry (IHC-P). View Larger

Decorin in Human Breast. Decorin was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; (CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to intralobular stromal tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human Decorin antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Decorin by Simple WesternTM. Simple Western lane view shows lysates of human uterus and human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 140-149 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry/ Immunofluorescence Detection of Human Decorin by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Human Decorin by Immunocytochemistry/ Immunofluorescence Immunohistochemical DCN expression in HSc and site-matched NS from burn patients.(a) Immunohistochemistry using a polyclonal goat anti-human DCN antibody and Alexa Fluor 488 secondary antibody (green fluorescence), and counterstained with DAPI (blue fluorescence) in representative site-matched sections of NS and HSc (scale bar = 50 μm). (b) Relative expression of DCN in matched superficial and deep NS and HSc sections was calculated from fluorescence using ImageJ (mean ± SEM, n = 4 patients, * P < 0.001). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0123054), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human Decorin by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Human Decorin by Immunocytochemistry/ Immunofluorescence Immunohistochemical DCN expression in HSc and site-matched NS from burn patients.(a) Immunohistochemistry using a polyclonal goat anti-human DCN antibody and Alexa Fluor 488 secondary antibody (green fluorescence), and counterstained with DAPI (blue fluorescence) in representative site-matched sections of NS and HSc (scale bar = 50 μm). (b) Relative expression of DCN in matched superficial and deep NS and HSc sections was calculated from fluorescence using ImageJ (mean ± SEM, n = 4 patients, * P < 0.001). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0123054), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Decorin

Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10‑12 tandem leucine-rich repeats (LRR) (1, 2). The human Decorin cDNA encodes a 359 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide. The 329 aa mature protein contains twelve LRR. Alternate splicing generates five isoforms with variable length deletions (3). Mature human and mouse Decorin share 80% aa sequence identity. In Decorin, serine 34 in the N-terminal domain is O-glycosylated. Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa. Decorin binds to fibronectin, TGF-beta, and type I and type II collagens. The binding of Decorin to various molecules was reported to be mediated via the core protein. Decorin has been implicated in matrix assembly and has also been reported to suppress the growth of various tumor cell lines by activating the epidermal growth factor receptor.

References
  1. Naito, Z. (2005) J. Nippon Med. Sch. 72:137.
  2. Matsushima, N. et al. (2005) Cell. Mol. Life Sci. 62:2771.
  3. Danielson, K. et al. (1993) Genomics 15:146.
Entrez Gene IDs
1634 (Human); 13179 (Mouse)
Alternate Names
Bone proteoglycan II; CSCD; DCN; decorin proteoglycan; Decorin; dermatan sulphate proteoglycans II; DSPG2; PG40; PGII; PG-II; PGS2; PG-S2; proteoglycan core protein; SLRR1B; SLRR1BPGS2; small leucine-rich protein 1B

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Citations for Human Decorin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. Development of stromal differentiation patterns in heterotypical models of artificial corneas generated by tissue engineering
    Authors: Blanco-Elices C, Morales-�lvarez C, Chato-Astrain J et al.
    Frontiers in Bioengineering and Biotechnology
  2. An immunosuppressive vascular niche drives macrophage polarization and immunotherapy resistance in glioblastoma
    Authors: Yang, F;Akhtar, MN;Zhang, D;El-Mayta, R;Shin, J;Dorsey, JF;Zhang, L;Xu, X;Guo, W;Bagley, SJ;Fuchs, SY;Koumenis, C;Lathia, JD;Mitchell, MJ;Gong, Y;Fan, Y;
    Science advances
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Development of stromal differentiation patterns in heterotypical models of artificial corneas generated by tissue engineering
    Authors: Blanco-Elices C, Morales-�lvarez C, Chato-Astrain J et al.
    Frontiers in Bioengineering and Biotechnology
  4. A single-cell comparison of adult and fetal human epicardium defines the age-associated changes in epicardial activity
    Authors: Vincent R. Knight-Schrijver, Hongorzul Davaapil, Semih Bayraktar, Alexander D. B. Ross, Kazumasa Kanemaru, James Cranley et al.
    Nature Cardiovascular Research
  5. Leptolysin, a Leptospira secreted metalloprotease of the pappalysin family with broad-spectrum activity
    Authors: Daniella dos Santos Courrol, Cristiane Castilho Fernandes da da Silva, Luan Gavião Prado, Rosa Maria Chura-Chambi, Ligia Morganti, Gisele Oliveira de Souza et al.
    Frontiers in Cellular and Infection Microbiology
  6. Reciprocal interplay between asporin and decorin: Implications in gastric cancer prognosis
    Authors: D Basak, Z Jamal, A Ghosh, PK Mondal, P Dey Talukd, S Ghosh, B Ghosh Roy, R Ghosh, A Halder, A Chowdhury, GK Dhali, BK Chattopadh, ML Saha, A Basu, S Roy, C Mukherjee, NK Biswas, U Chatterji, S Datta
    PLoS ONE, 2021-08-11;16(8):e0255915.
    Species: Human
    Sample Types: Tissue Homogenaets, Tissue Homogenates
    Applications: Co-IP, Western Blot
  7. CHPF Regulates the Aggressive Phenotypes of Hepatocellular Carcinoma Cells via the Modulation of the Decorin and TGF-&beta Pathways
    Authors: CH Liu, BR Wu, YJ Ho, YH Chu, WC Hsu, TJ Tseng, JP Li, WC Liao
    Cancers, 2021-03-12;13(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases
    Authors: AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano
    Sci Rep, 2020-07-31;10(1):12912.
    Species: Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  9. Comparison of the Opn-CreER and Ck19-CreER Drivers in Bile Ducts of Normal and Injured Mouse Livers
    Authors: Bram Lesaffer, Elisabeth Verboven, Leen Van Huffel, Iván M. Moya, Leo A. van Grunsven, Isabelle A. Leclercq et al.
    Cells
  10. Absence of the proteoglycan decorin reduces glucose tolerance in overfed male mice
    Authors: J Svärd, TH Røst, CEN Sommervoll, C Haugen, OA Gudbrandse, AE Mellgren, E Rødahl, J Fernø, SN Dankel, JV Sagen, G Mellgren
    Sci Rep, 2019-03-15;9(1):4614.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. Osteoblasts are "educated" by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment
    Authors: AD Kolb, AB Shupp, D Mukhopadhy, FC Marini, KM Bussard
    Breast Cancer Res., 2019-02-27;21(1):31.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Functional Assay, IHC-P
  12. Regulation of myelination by exosome associated retinoic acid release from NG2-positive cells
    Authors: MB Goncalves, Y Wu, E Clarke, J Grist, C Hobbs, D Trigo, J Jack, JPT Corcoran
    J. Neurosci., 2019-02-13;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Single-cell analysis of progenitor cell dynamics and lineage specification in the human fetal kidney.
    Authors: Menon R, Otto E, Kokoruda A, Zhou J, Zhang Z, Yoon E, Chen Y, Troyanskaya O, Spence J, Kretzler M, Cebrian C
    Development, 2018-08-30;145(16):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  14. Adenovirus-mediated decorin expression induces cancer cell death through activation of p53 and mitochondrial apoptosis
    Authors: AR Yoon, J Hong, CO Yun
    Oncotarget, 2017-09-08;8(44):76666-76685.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. In vitro characterization of a nanostructured fibrin agarose bio-artificial nerve substitute
    Authors: Víctor Carriel, Giuseppe Scionti, Fernando Campos, Olga Roda, Begoña Castro, Maria Cornelissen et al.
    Journal of Tissue Engineering and Regenerative Medicine
  16. Loss-of-function mutations in the X-linked biglycan gene cause a severe syndromic form of thoracic aortic aneurysms and dissections
    Authors: Josephina A.N. Meester, Geert Vandeweyer, Isabel Pintelon, Martin Lammens, Lana Van Hoorick, Simon De Belder et al.
    Genetics in Medicine
  17. Glucosamine exposure reduces proteoglycan synthesis in primary human endothelial cells in vitro
    Food Nutr Res, 2016-09-23;60(0):32615.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  18. Maternal obesity drives functional alterations in uterine NK cells
    JCI Insight, 2016-07-21;1(11):e85560.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. MicroRNA 181b regulates decorin production by dermal fibroblasts and may be a potential therapy for hypertrophic scar.
    Authors: Kwan P, Ding J, Tredget E
    PLoS ONE, 2015-04-02;10(4):e0123054.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. Bioactive IGF-1 release from collagen–GAG scaffold to enhance cartilage repair in vitro
    Authors: Leanne M. Mullen, Serena M. Best, Siddhartha Ghose, John Wardale, Neil Rushton, Ruth E. Cameron
    Journal of Materials Science: Materials in Medicine
  21. Probing the acceptor active site organization of the human recombinant beta1,4-galactosyltransferase 7 and design of xyloside-based inhibitors.
    Authors: Saliba M, Ramalanjaona N, Gulberti S, Bertin-Jung I, Thomas A, Dahbi S, Lopin-Bon C, Jacquinet J, Breton C, Ouzzine M, Fournel-Gigleux S
    J Biol Chem, 2015-01-07;290(12):7658-70.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Resistance of corneal RFUVA-cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases.
    Authors: Zhang Y, Mao X, Schwend T, Littlechild S, Conrad G
    Invest Ophthalmol Vis Sci, 2013-02-05;54(2):1014-25.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  23. Effects of Ultraviolet-A and Riboflavin on the Interaction of Collagen and Proteoglycans during Corneal Cross-linking.
    Authors: Zhang Y, Conrad AH, Conrad GW
    J. Biol. Chem., 2011-02-18;286(15):13011-22.
    Species: Bovine
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  24. Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1.
    Authors: Meen AJ, Oynebraten I, Reine TM, Duelli A, Svennevig K, Pejler G, Jenssen T, Kolset SO
    J. Biol. Chem., 2010-11-12;286(4):2636-47.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Adenovirus-mediated gene transfer of fibromodulin inhibits neointimal hyperplasia in an organ culture model of human saphenous vein graft disease.
    Authors: Ranjzad P, Salem HK, Kingston PA
    Gene Ther., 2009-05-28;16(9):1154-62.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  26. Syndromic and non-syndromic aneurysms of the human ascending aorta share activation of the Smad2 pathway.
    Authors: Gomez D, Al Haj Zen A, Borges LF, Philippe M, Gutierrez PS, Jondeau G, Michel JB, Vranckx R
    J. Pathol., 2009-05-01;218(1):131-42.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  27. Dermatan sulfate proteoglycan biglycan as a potential selectin L/CD44 ligand involved in selective recruitment of peripheral blood CD16(-) natural killer cells into human endometrium.
    Authors: Kitaya K, Yasuo T
    J. Leukoc. Biol., 2008-12-16;85(3):391-400.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  28. Decorin is a secreted protein associated with obesity and type 2 diabetes.
    Authors: Bolton K, Segal D, McMillan J, Jowett J, Heilbronn L, Abberton K, Zimmet P, Chisholm D, Collier G, Walder K
    Int J Obes (Lond), 2008-04-15;32(7):1113-21.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  29. Periodontitis destructions are restored by synthetic glycosaminoglycan mimetic.
    Authors: Lallam-Laroye C, Escartin Q, Zlowodzki AS, Barritault D, Caruelle JP, Baroukh B, Saffar JL, Colombier ML
    J Biomed Mater Res A, 2006-12-01;79(3):675-83.
    Species: Hamster
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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