Human DECR1 Antibody

Catalog #: AF7976 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7976
AF7976-SP

Detection of Human DECR1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DECR1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human DECR1 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human DECR1
Met233-Ser335
Accession # Q16698

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human DECR1 by Western Blot. Western blot shows lysates of human kidney tissue, human pancreas tissue, and human liver tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human/Mouse/Rat DECR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7976) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DECR1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DECR1

DECR-1 (2, 4-DiEnol CoA Reductase, mitochondrial) is a 33-36 kDa member of the DECR subfamily, short-chain dehydrogenase/reductase family of molecules. It is a mitochondrial enzyme that is expressed in multiple cell types, particularly those involved in oxidative degradation of fatty acids. Fatty acids provide energy following their degradation by four enzymes that comprise the beta -oxidation cycle. The optimal configuration for fatty acids in this cycle is one of saturation. However, many fatty acids that appear before this cycle are unsaturated, and it is necessary to transform them into a suitable configuration for processing. DECR-1 converts a two double-bonded substrate into a one double-bonded substrate, which subsequently undergoes isomerization and entry into the beta -oxidation cycle. Human DECR-1 is 335 amino acids (aa) in length. It contains a mitochondrial targeting sequence (aa 1-34), and a 301 aa mature region that possesses one catalytic site (aa 57-302). There are two potential acetylation sites at Lys110 and Lys230. DECR-1 forms homotetramers. One potential isoform variant shows an alternative start site at Met93 coupled to a deletion of aa 292-335. Over aa 233-335, human DECR-1 shares 87% aa sequence identity with mouse DECR-1.

Long Name

2,4-Dienoyl CoA Reductase 1, Mitochondrial

Entrez Gene IDs

1666 (Human); 67460 (Mouse); 117543 (Rat)

Alternate Names

2,4-dienoyl CoA reductase 1, mitochondrial; 4-enoyl-CoA reductase [NADPH]; 4-enoyl-CoA reductase; DECR; DECR1; DECR2,4-dienoyl-CoA reductase [NADPH]2,4-dienoyl-CoA reductase, mitochondrial; EC 1.3.1.34; NADPH; SDR18C1; short chain dehydrogenase/reductase family 18C, member 1