Human DEK Antibody Summary
Val282-Ser375
Accession # P35659
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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DEK in HeLa Human Cell Line. DEK was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human DEK Monoclonal Antibody (Catalog # MAB70201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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DEK in Human Cervical Cancer Tissue. DEK was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Mouse Anti-Human DEK Monoclonal Antibody (Catalog # MAB70201) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DEK
The DEK oncogene is an approximately 45 kDa phosphoprotein with pleiotropic activities. It is a chromatin remodeling factor, a transcriptional coactivator and corepressor, a component of RNA spliceosomes, and a promoter of DNA repair during apoptosis. DEK is upregulated in many cancers and promotes epithelial cell transformation. It functions as a proinflammatory factor when secreted by activated macrophages. It is an autoantigen in juvenile arthritis and lupus and a target of chromosomal translocation with the CAN gene in acute myelogenous leukemia. DEK contains one SAP domain (aa 149-183), one nuclear localization sequence (aa 205-221), and a DNA-binding C-terminal domain (aa 321-375). Within aa 292-375, human DEK shares 95% aa sequence identity with mouse and rat DEK.
Product Datasheets
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