Human DEP-1/CD148 Antibody

Catalog #: MAB19341 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB19341
MAB19341-SP

All DEP-1/CD148 Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DEP-1/CD148 Antibody Summary

Species Reactivity

Human

Specificity

Detects human DEP‑1/CD148.

Source

Monoclonal Mouse IgG_2B Clone # 261922

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human DEP‑1/CD148
Arg997-Ala1337
Accession # Q12913

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Human whole blood

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DEP-1/CD148

Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kilodalton) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T-cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta‑catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain.

References

Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680.
Sorby, M. et al. (2001) Oncogene 20:5219.
Jandt, E. et al. (2003) Oncogene 22:4175.
Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793.
Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393.
Borges, L.G. et al. (1996) Circ. Res. 79:570.
de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800.
Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295.
Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817.
Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728.
Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219.
Holsinger, L.J. et al. (2002) Oncogene 21:7067.

Long Name

Density Enhanced Receptor Protein Tyrosine Phosphatase

Entrez Gene IDs

5795 (Human); 19271 (Mouse); 29645 (Rat)

Alternate Names

CD148 antigen; CD148; Density-enhanced phosphatase 1; DEP1; DEP-1; DEP1EC 3.1.3.48; HPTP eta; HPTPeta; HPTP-eta; human density enhanced phosphatase-1; protein tyrosine phosphatase, receptor type, J polypeptide; protein tyrosine phosphatase, receptor type, J; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; PTPRJ; receptor-type tyrosine-protein phosphatase eta; R-PTP-ETA; R-PTP-J; SCC1; susceptibility to colon cancer 1, mouse, homolog of