Human Desmocollin-2 Biotinylated Antibody

Catalog #: BAF4688 Datasheet / COA / SDS

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BAF4688

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Human Desmocollin-2 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Desmocollin-2 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) Desmocollin-1 and rhDesmocollin-3 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Desmocollin-2
Arg136-Arg684
Accession # Q02487

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Desmocollin‑2 (Catalog # 4688-DC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desmocollin-2

Desmocollin-2 (DSC2) is a 100-110 kDa transmembrane glycoprotein member of the cadherin family of calcium dependent adhesion molecules (1 - 3). Human DSC2 is synthesized as a 901 amino acid (aa) precursor. It contains a 108 aa propeptide plus a mature region that consists of a 559 aa extracellular domain (ECD) that contains five cadherin-like domains, a 21 aa transmembrane segment, and a 186 aa cytoplasmic region (4). Within the ECD, human DSC2 shares 74% - 79% aa sequence identity with bovine, mouse, and rat DSC2. It shares 54% and 64% aa sequence identity with Desmocollin-1 and -3, respectively. DSC2 is also expressed as long and short splice forms which differ in their cytoplasmic regions (4, 5). The N-terminal two cadherin-like domains mediate homophilic interactions as well as heterophilic interactions with Desmoglein-2 (6). DSC2 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues (1, 5, 7). In contrast to DSC1 and DSC3, DSC2 is also expressed in simple epithelia lining the gastrointestinal tract, liver, and kidney (5). During colon carcinogenesis however, DSC2 is downregulated, while DSC1 and DSC3 are upregulated (8). DSC2 is additionally expressed in the myocardium, and a variety of DSC2 mutations are associated with the cardiac arrhythmia disorder ARVC (5, 9).

References

Kottke, M.D. et al. (2006) J. Cell Sci. 119:797.
Garrod, D.R. et al. (2002) Mol. Membrane Biol. 19:81.
Leckband, D. and A. Prakasam (2006) Annu. Rev. Biomed. Eng. 8:259.
Parker, A.E. et al. (1991) J. Biol. Chem. 266:10438.
Nuber, U.A. et al. (1995) Eur. J. Cell Biol. 66:69.
Syed, S. et al. (2002) Biochem. J. 362:317.
Theis, D.G. et al. (1993) Int. J. Dev. Biol. 37:101.
Khan, K. et al. (2006) Br. J. Cancer 95:1367.
van Tintelen, J.P. et al. (2007) Curr. Opin. Cardiol. 22:185.

Entrez Gene IDs

1824 (Human); 13506 (Mouse); 291760 (Rat)

Alternate Names

ARVD11; Cadherin family member 2; CDHF2; CDHF2DGII/III; desmocollin 2; Desmocollin2; Desmocollin-2; Desmocollin-3; Desmosomal glycoprotein II; desmosomal glycoprotein II/III; Desmosomal glycoprotein III; DG2; DSC2; DSC3DKFZp686I11137