Human Desmocollin-3 Antibody Summary
Arg136-Lys690
Accession # Q14574
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Desmocollin-3
Desmocollin-3 is a 100‑110 kDa type I transmembrane glycoprotein that belongs to the cadherin family of calcium dependent adhesion molecules (1‑3). The human Desmocollin-3 is synthesized with a 108 amino acid (aa) propeptide. The mature protein consists of a 555 aa extracellular domain (ECD) that contains five cadherin‑like domains, a 21 aa transmembrane segment, and a 185 aa cytoplasmic domain (4, 5). Within the ECD, human Desmocollin-3 shares 78% aa sequence identity with mouse and rat Desmocollin-3. It shares 52% and 64% aa sequence identity with Desmocollin-1 and -2, respectively. A splice variant of human Desmocollin-3 has a truncated and substituted cytoplasmic domain (4, 6). Desmocollin-3 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues (5, 7, 8). Desmocollin-1 and -2, by contrast, are preferentially localized in the more superficial layers (7). In mouse, Desmocollin-3 is expressed throughout embryogenesis and is required for non‑desmosomal functions during early development (9, 10). During apoptosis, both isoforms of Desmocollin-3 are proteolytically cleaved by caspases and metalloproteases (11). Desmocollin-3 can be up‑regulated or down‑regulated in carcinoma (6, 12, 13).
- Kottke, M.D. et al. (2006) J. Cell Sci. 119:797.
- Garrod, D.R. et al. (2002) Mol. Memb. Biol. 19:81.
- Leckband, D. and A. Prakasam (2006) Annu. Rev. Biomed. Eng. 8:259.
- Kawamura, K. et al. (1994) J. Biol. Chem. 269:26295.
- King, I.A. et al. (1995) J. Invest. Dermatol. 105:314.
- Klus, G.T. et al. (2001) Int. J. Oncol. 19:169.
- North, A.J. et al. (1996) Proc. Natl. Acad. Sci. 93:7701.
- Nuber, U.A. et al. (1996) Eur. J. Cell Biol. 71:1.
- Chidgey, M.A.J. et al. (1997) Dev. Dyn. 210:315.
- Den, Z. et al. (2006) J. Cell Sci. 119:482.
- Weiske, J. et al. (2001) J. Biol. Chem. 276:41175.
- Wang, L. et al. (2007) Virchows Arch. Sept. 11 epub.
- Boelens, M.C. et al. (2007) J. Clin. Pathol. 60:608.
Product Datasheets
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Desmocollin-3 Antibody
Average Rating: 5 (Based on 1 Review)
Have you used Human Desmocollin-3 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
