Human Desmoglein-2 Antibody Summary
Met1-Gly608
Accession # CAA81226
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Desmoglein‑2 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Desmoglein-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF947) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Desmoglein-2 at approximately 90-160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Desmoglein‑2 by Simple WesternTM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Desmoglein-2 at approximately 159-164 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Desmoglein-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF947) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Desmoglein-2
Desmoglein-2 is one of three members of the desmoglein subfamily of calcium-dependent cadherin cell adhesion molecules. Together with desmocollins, another subfamily within the cadherin superfamily, the desmoglein isoforms form the adhesive components of desmosomes, the cell-cell adhesive structures that are found in epithelial cells. Human Desmoglein-2 is a type I transmembrane glycoprotein of 1117 amino acid (aa) residues with a 23 aa signal peptide and a 25 aa propeptide. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Instead of having the HAV adhesion motif found in type I cadherins, desmogleins have R/YAL as the adhesion motif on its amino-terminal cadherin repeat. The cytoplasmic tails of desmogleins interact with desmoplakins, plakoglobin and plakophilins. In turn, these proteins link the desmogleins with the intermediate filaments. Desmoglein-2 has been shown to be important in establishing cell-cell adhesion and function in epithelial cells. Desmoglein-2 was originally identified in colon carcinoma and colon, and was named HDGC (human desmoglein colon).
- Nollet, R. et al. (2000) J. Mol. Biol. 299:551.
- Elias, P. et al. (2001) J. Cell Biol. 153:243.
- Arnemann, J. et al. (1992) Genomics 13:484.
Product Datasheets
Citations for Human Desmoglein-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
14
Citations: Showing 1 - 10
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Desmoglein-2 is important for islet function and beta-cell survival
Authors: KK Myo Min, D Rojas-Cana, D Penko, M DeNichilo, MP Cockshell, CB Ffrench, EJ Thompson, O Asplund, CJ Drogemulle, RB Prasad, L Groop, ST Grey, HE Thomas, T Loudovaris, TW Kay, MG Mahoney, CF Jessup, PT Coates, CS Bonder
Cell Death & Disease, 2022-10-29;13(10):911.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
High frequency of anti-DSG 2 antibodies in post COVID-19 serum samples
Authors: Edward C.Y. Lee, Ryan E. Tyler, Derrick Johnson, Natalie Koh, Biauw Chi Ong, Shi Yin Foo et al.
Journal of Molecular and Cellular Cardiology
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In vivo HSC transduction in rhesus macaques with an HDAd5/3+ vector targeting desmoglein 2 and transiently overexpressing cxcr4
Authors: Hongjie Wang, Audrey Germond, Chang Li, Sucheol Gil, Jiho Kim, Hans-Peter Kiem et al.
Blood Advances
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Translational development of a tumor junction opening technology
Authors: J Kim, C Li, H Wang, S Kaviraj, S Singh, L Savergave, A Raghuwansh, S Gil, A Germond, A Baldessari, B Chen, S Roffler, P Fender, C Drescher, D Carter, A Lieber
Scientific Reports, 2022-05-11;12(1):7753.
Species: Cynomolgus Monkey
Sample Types: Whole Tissue
Applications: IHC -
Desmoglein-2 as a prognostic and biomarker in ovarian cancer
Authors: Kim J, Beidler P, Wang H et al.
Cancer Biology & Therapy
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The cell–cell junctions of mammalian testes. III. Absence of an endothelial cell layer covering the peritubular wall of the seminiferous tubules—an immunocytochemical correction of a 50-year-old error in the literature
Authors: Werner W. Franke, Lisa M. Domke, Yvette Dörflinger, Ralf Zimbelmann
Cell and Tissue Research
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Kempopeptin C, a Novel Marine-Derived Serine Protease Inhibitor Targeting Invasive Breast Cancer
Authors: Fatma H. Al-Awadhi, Lilibeth A. Salvador, Brian K. Law, Valerie J. Paul, Hendrik Luesch
Marine Drugs
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Studies on the Interaction of Tumor-Derived HD5 Alpha Defensins with Adenoviruses and Implications for Oncolytic Adenovirus Therapy
Authors: Charles Vragniau, Jens-Martin Hübner, Peter Beidler, Sucheol Gil, Kamola Saydaminova, Zhuo-Zhuang Lu et al.
Journal of Virology
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Intracellular Signaling and Desmoglein 2 Shedding Triggered by Human Adenoviruses Ad3, Ad14, and Ad14P1
Authors: Hongjie Wang, Corinne Ducournau, Kamola Saydaminova, Maximilian Richter, Roma Yumul, Martin Ho et al.
Journal of Virology
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Preclinical safety and efficacy studies with an affinity-enhanced epithelial junction opener and PEGylated liposomal doxorubicin
Authors: Maximilian Richter, Roma Yumul, Hongjie Wang, Kamola Saydaminova, Martin Ho, Drew May et al.
Molecular Therapy - Methods & Clinical Development
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Penton-dodecahedral particles trigger opening of intercellular junctions and facilitate viral spread during adenovirus serotype 3 infection of epithelial cells.
Authors: Lu Z, Wang H, Zhang Y, Cao H, Li Z, Fender P, Lieber A
PLoS Pathog, 2013-10-31;9(10):e1003718.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
A new human DSG2-transgenic mouse model for studying the tropism and pathology of human adenoviruses.
Authors: Wang H, Beyer I, Persson J, Song H, Li Z, Richter M, Cao H, van Rensburg R, Yao X, Hudkins K, Yumul R, Zhang XB, Yu M, Fender P, Hemminki A, Lieber A
J. Virol., 2012-03-28;86(11):6286-302.
Species: Human, Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Tissue
Applications: IHC-Fr, IHC-P -
Chromatin structure of two genomic sites for targeted transgene integration in induced pluripotent stem cells and hematopoietic stem cells.
Authors: van Rensburg, R, Beyer, I, Yao, X-Y, Wang, H, Denisenko, O, Li, Z-Y, Russell, D W, Miller, D G, Gregory, P, Holmes, M, Bomsztyk, K, Lieber, A
Gene Ther, 2012-03-22;20(2):201-14.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Multimerization of adenovirus serotype 3 fiber knob domains is required for efficient binding of virus to desmoglein 2 and subsequent opening of epithelial junctions.
Authors: Wang H, Li Z, Yumul R, Lara S, Hemminki A, Fender P, Lieber A
J. Virol., 2011-04-27;85(13):6390-402.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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