Human Desmoglein-3 Antibody Summary
Glu50-Arg615
Accession # P32926
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Desmoglein‑3 in Human Squamous Cell Carcinoma. Desmoglein-3 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Desmoglein-3 Monoclonal Antibody (Catalog # MAB1720) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Desmoglein-3
Desmoglein-3, also known as pemphigus vulgaris antigen (PVA), is a 130 kDa transmembrane glycoprotein that belongs to the cadherin family of calcium dependent adhesion molecules (1‑3). Human Desmoglein-3 is synthesized with a 26 amino acid (aa) propeptide. The mature protein consists of a 566 aa extracellular domain (ECD) that contains four cadherin-like domains, a 25 aa transmembrane segment, and a 359 aa cytoplasmic domain (4). Within the ECD, human Desmoglein-3 shares 78% aa sequence identity with mouse and rat Desmoglein-3. It shares 44%, 47%, and 56% aa sequence identity with Desmoglein-1, -2, and -4 respectively. Desmoglein-3 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues (4‑6). During apoptosis, Desmoglein-3 is cleaved by caspases, plus MMP-2, and MMP-9 at sites within the cytoplasmic and extracellular regions, resulting in shortened transmembrane forms and a soluble 75 kDa ECD fragment (7, 8). The downregulation of Desmoglein-3 in oral squamous cell carcinoma correlates with metastatic potential (9). Desmoglein-3 is the target of autoantibodies in pemphigus vulgaris, a blistering skin disorder with compromised inter-keratinocyte adhesion (6, 10). Binding of these antibodies triggers Desmoglein-3 internalization and degradation by keratinocytes (10).
- Kottke, M.D. et al. (2006) J. Cell Sci. 119:797.
- Garrod, D.R. et al. (2002) Mol. Membr. Biol. 19:81.
- Leckband, D. and A. Prakasam (2006) Annu. Rev. Biomed. Eng. 8:259.
- Amagai, M. et al. (1991) Cell 67:869.
- Silos, S.A. et al. (1996) J. Biol. Chem. 271:17504.
- Amagai, M. et al (1996) J. Invest. Dermatol. 106:351.
- Weiske, J. et al. (2001) J. Biol. Chem. 276:41175.
- Cirillo, N. et al. (2007) Oral Dis. 13:341.
- Wang, L. et al. (2007) Virchows Arch. Sept. 11 epub.
- Calkins, C.C. et al. (2006) J. Biol. Chem. 281:7623.
Product Datasheets
Citations for Human Desmoglein-3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Detecting cancer metastasis and accompanying protein biomarkers at single cell levels using a 3D-printed microfluidic immunoarray
Authors: Mohamed Sharafeldin, Tianqi Chen, Gulsum Ucak Ozkaya, Dharamainder Choudhary, Alfredo A. Molinolo, J. Silvio Gutkind et al.
Biosensors and Bioelectronics
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Oncoproteomic and gene expression analyses identify prognostic biomarkers for second primary malignancy in patients with head and neck squamous cell carcinoma
Authors: S Bunbanjerd, N Vorasan, T Saethang, T Pongrujiko, D Pangpunyak, N Mongkonsir, L Arsa, N Thokanit, W Pongsapich, T Anekpurita, N Ngamphaibo, A Jinawath, S Sunpawerav, T Pisitkun, B Suktitipat, N Jinawath
Mod. Pathol., 2019-02-08;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Streptococcal Cysteine Protease-Mediated Cleavage of Desmogleins Is Involved in the Pathogenesis of Cutaneous Infection
Authors: T Sumitomo, Y Mori, Y Nakamura, M Honda-Ogaw, S Nakagawa, M Yamaguchi, H Matsue, Y Terao, M Nakata, S Kawabata
Front Cell Infect Microbiol, 2018-01-24;8(0):10.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot -
Gene expression profiling of idiopathic interstitial pneumonias (IIPs): identification of potential diagnostic markers and therapeutic targets
Authors: Y Horimasu, N Ishikawa, M Taniwaki, K Yamaguchi, K Hamai, H Iwamoto, S Ohshimo, H Hamada, N Hattori, M Okada, K Arihiro, Y Ohtsuki, N Kohno
BMC Med. Genet., 2017-08-18;18(1):88.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Central role of alpha9 acetylcholine receptor in coordinating keratinocyte adhesion and motility at the initiation of epithelialization.
Authors: Chernyavsky AI, Arredondo J, Vetter DE, Grando SA
Exp. Cell Res., 2007-07-20;313(16):3542-55.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Desmoglein versus non-desmoglein signaling in pemphigus acantholysis: characterization of novel signaling pathways downstream of pemphigus vulgaris antigens.
Authors: Chernyavsky AI, Arredondo J, Kitajima Y, Sato-Nagai M, Grando SA
J. Biol. Chem., 2007-03-07;282(18):13804-12.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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