Human DFF45/ICAD Antibody

Catalog #: MAB4679 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4679
MAB4679-SP

Detection of Human DFF45/ICAD by Western Blot.

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All DFF45/ICAD Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DFF45/ICAD Antibody Summary

Species Reactivity

Human

Specificity

Detects human DFF45/ICAD in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 495403

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human DFF45/ICAD
Met1-Thr331 (predicted)
Accession # O00273

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human DFF45/ICAD by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and A549 human lung carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human DFF45/ICAD Monoclonal Antibody (Catalog # MAB4679) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DFF45/ICAD at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DFF45/ICAD

DFF45 (DNA fragmentation factor 45 kDa subunit, also known as ICAD) is a 45 kDa member of the CIDE domain-containing family of proteins. Human DFF45 is 331 amino acids (aa) in length. It contains an N-terminal CIDE domain (aa 17‑96) plus two caspase-3 cleavage sites (aa’s 117‑118 and 224‑225). DFF45 is a cytoplasmic protein that inhibits DFF40 DNAase. Normally, DFF45 and DFF40 form an enzymatically-inactive noncovalent heterodimer. Upon activation of the apoptotic cascade, DFF45 is cleaved into three fragments. These dissociate from DFF40 and induce DFF40 oligomerization and activation. Human DFF45 has two potential splice forms. One is truncated, and shows a seven aa substitution for aa 262‑331; a second is extended, and shows a 45 aa substitution for the C-terminal five amino acids. Human DFF45 is 76% aa identical to mouse DFF45.

Long Name

DNA Fragmentation Factor, Subunit alpha

Entrez Gene IDs

1676 (Human); 13347 (Mouse); 114214 (Rat)

Alternate Names

DFF1; DFF1DNA fragmentation factor 45 kDa subunit; DFF45; DFF45DNA fragmentation factor subunit alpha; DFF-45DNA fragmentation factor, 45 kD, alpha polypeptide; DFFA; DNA fragmentation factor, 45kDa, alpha polypeptide; ICAD; Inhibitor of CAD