Human Dishevelled-1 Antibody

Name: Human Dishevelled-1 Antibody
Brand: R&D Systems
SKU: AF3316
Rating: 3.7 (3 reviews)

Catalog #: AF3316
1 Citation 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3316
AF3316-SP

All Dishevelled-1 Antibodies

Product Details

Citations (1)

FAQs

Supplemental Products

Reviews (3)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Dishevelled-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Dishevelled-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) DVL‑2 and rhDVL‑3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Dishevelled-1
Arg150-Leu215
Accession # O14640

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Dishevelled‑1

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human skeletal muscle

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dishevelled-1

DVL-1 (Dishevelled-1) is a cytoplasmic phosphoprotein that is one of the three human orthologs of the Drosophila dishevelled segment polarity protein. Human DVL-1 is expressed in multiple tissues where it participates in Wnt signal transduction. DVL-1 is a modular protein containing DIX, PDZ and DEP linker regions that connect Wnt/Frizzled engagement to proteins involved in the inactivation of beta -catenin destruction in the Wnt canonical pathway. Human DVL-1 shows 93% aa identity with mouse and rat DVL-1 over the sequence used for immunization. Three shorter isoforms have been described, but it is unclear whether these are actually expressed.

Entrez Gene IDs

1855 (Human); 13542 (Mouse); 83721 (Rat)

Alternate Names

dishevelled 1 (homologous to Drosophila dsh); dishevelled, dsh homolog 1 (Drosophila); Dishevelled1; Dishevelled-1; DSH homolog 1; Dsh; DVL; DVL1; DVL1L1; MGC54245; segment polarity protein dishevelled homolog DVL-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Dishevelled-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

The noncanonical WNT pathway is operative in idiopathic pulmonary arterial hypertension.
Authors: Laumanns IP, Fink L, Wilhelm J, Wolff JC, Mitnacht-Kraus R, Graef-Hoechst S, Stein MM, Bohle RM, Klepetko W, Hoda MA, Schermuly RT, Grimminger F, Seeger W, Voswinckel R
Am. J. Respir. Cell Mol. Biol., 2008-11-21;40(6):683-91.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P