Human Dishevelled-2 Antibody

Catalog #: MAB4216
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4216
MAB4216-SP

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Dishevelled-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Dishevelled‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) DVL-1 or rhDVL-3 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 373413

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Dishevelled-2
Leu78-Thr250
Accession # O14641

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Dishevelled‑2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dishevelled-2

Dishevelled-2 (DVL-2) is a 90‑95 kDa cytoplasmic phosphoprotein that is one of the three Drosophila dishevelled orthologs. Human DVL-2 is expressed in multiple tissues, participates in Wnt signal transduction and may mediate Wnt-dependent motility. DVL-2 is a modular protein containing DIX, PDZ and DEP linker regions that connect Wnt/Frizzled engagement to proteins involved in the inactivation of beta -catenin destruction in the Wnt canonical pathway. Human and mouse DVL-2 share 94% aa identity over the sequence used for immunization.

Entrez Gene IDs

1856 (Human); 13543 (Mouse); 303251 (Rat)

Alternate Names

dishevelled 2 (homologous to Drosophila dsh); dishevelled, dsh homolog 2 (Drosophila); Dishevelled2; Dishevelled-2; DSH homolog 2; DVL2; segment polarity protein dishevelled homolog DVL-2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Dishevelled-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1