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Human DLL1 Antibody

Catalog #: AF1818
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1818
AF1818-SP
Detection of DLL1 in T98G Human Cell Line by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DLL1 Antibody Summary

Species Reactivity
Human
Specificity
Detects recombinant human DLL1 in direct ELISAs. In direct ELISAs, less than 1% cross‑reactivity with recombinant human DLL4 and recombinant mouse DLL4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DLL1
Ser22-Gly540
Accession # AAG09716
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
1 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of DLL1 antibody in T98G Human Cell Line antibody by Flow Cytometry. View Larger

Detection of DLL1 in T98G Human Cell Line by Flow Cytometry. T98G human glioblastoma cell line was stained with Goat Anti-Human DLL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1818, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLL1

Delta-like protein 1 (DLL1) is a 90-100 kDa type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6-11). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13).

References
  1. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785. 
  2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436. 
  3. Karanu, F.N. et al. (2001) Blood 97:1960. 
  4. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. 
  5. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499. 
  6. Takahashi, Y. et al. (2003) Development 130:4259. 
  7. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
  8. Kiernan, A.E. et al. (2005) Development 132:4353.
  9. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
  10. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
  11. Santos, M.A. et al. (2007) Proc. Natl. Acad. Sci. USA 104:15454.
  12. Limbourg, A. et al. (2007) Circ. Res. 100:363.
  13. Purow, B.W. et al. (2005) Cancer Res. 65:2353.
Long Name
Delta-like 1
Entrez Gene IDs
28514 (Human); 13388 (Mouse); 84010 (Rat)
Alternate Names
delta (Drosophila)-like 1; Delta 1; Delta; Delta1; delta-like 1 (Drosophila); delta-like protein 1; DL1; DLL1; Drosophila Delta homolog 1; H-Delta-1

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Citations for Human DLL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Selective CDK9 inhibition overcomes TRAIL resistance by concomitant suppression of cFlip and Mcl-1.
    Authors: Lemke J, von Karstedt S, Abd El Hay M, Conti A, Arce F, Montinaro A, Papenfuss K, El-Bahrawy M, Walczak H
    Cell Death Differ, 2013-12-20;21(3):491-502.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Roles of Pofut1 and O-fucose in mammalian Notch signaling.
    Authors: Stahl M, Uemura K, Ge C, Shi S, Tashima Y, Stanley P
    J. Biol. Chem., 2008-03-17;283(20):13638-51.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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