Human DLL4 PE-conjugated Antibody

Name: Human DLL4 PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB1506P
Rating: 4 (1 reviews)

Catalog #: FAB1506P 1 Review Datasheet / COA / SDS

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FAB1506P

Detection of DLL4 in HUVEC Human Cells by Flow Cytometry.

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Human DLL4 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human DLL4 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 447506

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DLL4
Ser27-Pro524
Accession # Q9NR61

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of DLL4 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Rat Anti-Human DLL4 PE-conjugated Monoclonal Antibody (Catalog # FAB1506P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: DLL4

Delta-like ligand 4 (DLL4) is a transmembrane protein that contains one DSL domain and eight tandem EGF-like repeats. DLL4 is expressed on arterial endothelial cells where it signals through Notch 1 and Notch 4. DLL4 expression is induced by VEGF and regulates the proliferation of endothelial tip cells during vascular sprouting. DLL4 blockade as well as overexpression can suppress tumor growth by promoting dysregulated angiogenesis. Within the extracellular domain, human DLL4 shares 85% amino acid sequence identity with mouse and rat DLL4.

Long Name

Delta-like 4

Entrez Gene IDs

54567 (Human); 54485 (Mouse); 100152163 (Porcine)

Alternate Names

Delta 4 precursor; delta 4; delta ligand 4; delta4; delta-like 4 (Drosophila); delta-like 4 homolog (Drosophila); delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; DLL4; Drosophila Delta homolog 4; hdelta2; MGC126344; notch ligand delta-2; notch ligand DLL4