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Human DLX5 Antibody

Catalog #: AF6710
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6710
AF6710-SP
DLX5 in Human Mesenchymal Stem Cells.
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Citations (1)
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Human DLX5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DLX5 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse DLX5 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human DLX5
Asn216-Tyr289
Accession # P56178
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry DLX5 antibody in Human Mesenchymal Stem Cells by Immunocytochemistry (ICC). View Larger

DLX5 in Human Mesenchymal Stem Cells. DLX5 was detected in immersion fixed human mesenchymal stem cells differentiated into osteocytes using Sheep Anti-Human DLX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6710) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLX5

DLX5 (distal-less homeobox 5) is a 33-35 kDa member of the distal-less homeobox family of transcription factors. It is expressed in embryonic mesenchyme where it promotes cartilage and bone formation, and in nervous tissue, where it promotes interneuron development. Human DLX5 is 289 amino acids (aa) in length. It contains one DNA-binding homeobox domain (aa 137-196), and two phosphorylation sites at Ser34 and Ser217 that, when utilized, increases transcriptional activity. DLX5 likely forms homodimers, and is known to heterodimerize with MSX-2. Over aa 216-289, human DLX5 shares 99% aa identity with mouse DLX5.

Long Name
Distal-less Homeobox 5
Entrez Gene IDs
1749 (Human); 13395 (Mouse); 25431 (Rat)
Alternate Names
distal-less homeo box 5; distal-less homeobox 5; DLX5; homeobox protein DLX-5

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Citation for Human DLX5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Hydrogel mechanics are a key driver of bone formation by mesenchymal stromal cell spheroids
    Authors: Jacklyn Whitehead, Katherine H. Griffin, Marissa Gionet-Gonzales, Charlotte E. Vorwald, Serena E. Cinque, J. Kent Leach
    Biomaterials

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