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Antibody

Human DNAM-1/CD226
Antibody

Catalog #: MAB6662 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6662-100
MAB6662-SP
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Human DNAM-1/CD226
Antibody Summary

Species Reactivity
Human
Specificity
Detects human DNAM-1/CD226 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 1035502
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell HEK293-derived human DNAM-1/CD226
Glu19-Asn247
Accession # Q15762
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human DNAM-1/CD226
Monoclonal Antibody (Catalog # MAB6663).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DNAM-1/CD226

DNAX Accessory Molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112 (3, 4). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (2-6), dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the formation of osteoclasts (7-10). Platelet-endothelium interactions mediated by DNAM-1 can enable the metastasis of tumor cells to the lung (11). CD96 competes with DNAM-1 for binding to CD155 and blocks DNAM-1 mediated NK cell activation (12). In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation (6, 9, 13-15).

References
  1. Zingoni, A. et al. (2013) Front. Immunol. 3:408.
  2. Shibuya, A. et al. (1996) Immunity 4:573.
  3. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
  4. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
  5. Dardalhon, V. et al. (2005) J. Immunol. 175:1558.
  6. Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.
  7. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
  8. Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
  9. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
  10. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
  11. Morimoto, K. et al. (2007) Oncogene 27:264.
  12. Chan, C.J. et al. (2014) Nat. Immunol. 15:431.
  13. Shibuya, K. et al. (1999) Immunity 11:615.
  14. Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.
  15. Shibuya, A. et al. (1998) J. Immunol. 166:1671.
Long Name
DNAX Accessory Molecule 1
Entrez Gene IDs
10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)
Alternate Names
CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1; DNAM-1; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1

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