Human DNAM-1/CD226 PE-conjugated Antibody Summary
Glu19-Asn247 (predicted)
Accession # Q15762
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of DNAM-1/CD226 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD3-cells were stained with Mouse Anti-Human DNAM-1/CD226 PE-conjugated Monoclonal Antibody (Catalog # FAB666P) and Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: DNAM-1/CD226
DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112 (3, 4). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (2‑6), dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the formation of osteoclasts (7‑10). Platelet-endothelium interactions mediated by DNAM-1 enable the metastasis of tumor cells to the lung (11). In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation (6, 9, 12‑14).
- Fuchs, A. and M. Colonna (2006) Semin. Cancer Biol. 16:359.
- Shibuya, A. et al. (1996) Immunity 4:573.
- Bottino, C. et al. (2003) J. Exp. Med. 198:557.
- Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
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- Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
- Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
- Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
- Morimoto, K. et al. (2007) Oncogene July 16 epub.
- Shibuya, K. et al. (1999) Immunity 11:615.
- Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.
- Shibuya, A. et al. (1998) J. Immunol. 166:1671.
Product Datasheets
Citations for Human DNAM-1/CD226 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Expanded and activated allogeneic NK cells are cytotoxic against B-chronic lymphocytic leukemia (B-CLL) cells with sporadic cases of resistance
Authors: T Calvo, C Reina-Orti, D Giraldos, M Gascón, D Woods, J Asenjo, J Marco-Brua, G Azaceta, I Izquierdo, L Palomera, D Sánchez-Ma, I Marzo, J Naval, C Vilches, M Villalba, A Anel
Sci Rep, 2020-11-10;10(1):19398.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-12, IL-15, and IL-18 pre-activated NK cells target resistant T cell acute lymphoblastic leukemia and delay leukemia development in vivo
Authors: M Boieri, A Ulvmoen, A Sudworth, C Lendrem, M Collin, AM Dickinson, L Kveberg, M Inngjerdin
Oncoimmunology, 2017-01-17;6(3):e1274478.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Dopamine inhibits the effector functions of activated NK cells via the upregulation of the D5 receptor.
Authors: Mikulak J, Bozzo L, Roberto A, Pontarini E, Tentorio P, Hudspeth K, Lugli E, Mavilio D
J Immunol, 2014-08-15;193(6):2792-800.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The decreased expression of Siglec-7 represents an early marker of dysfunctional natural killer-cell subsets associated with high levels of HIV-1 viremia.
Authors: Brunetta E, Fogli M, Varchetta S, Bozzo L, Hudspeth KL, Marcenaro E, Moretta A, Mavilio D
Blood, 2009-08-26;114(18):3822-30.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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