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Human DNER Biotinylated Antibody

Catalog #: BAF3646
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BAF3646
Detection of Human DNER by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DNER Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human DNER in Western blots. In Western blots, approximately 30% cross-reactivity with recombinant mouse DNER is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DNER
Arg29-His637
Accession # Q8NFT8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of mouse embryo (E13.5)
Immunocytochemistry
5-15 µg/mL
Immersion fixed A172 human glioblastoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human DNER antibody by Western Blot. View Larger

Detection of Human DNER by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DNER Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3646) followed by streptavidin-HRP. A specific band was detected for DNER at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DNER

DNER (delta and notch-like EGF-related receptor; also BET) is a 120-150 kDa type I transmembrane glycoprotein that is reminescent of notch ligands such as Delta and Jagged. It is found on neuron dendrites and cell bodies but not on axons. Human DNER is 703 amino acids (aa) in length, and contains a 606 aa extracellular domain (ECD) (aa 35-640) plus a 76 aa cytoplasmic region. The ECD contains nine consecutive EGF-like domains, followed by one follistatin-like domain and a tenth EGF-like domain. DNER binding to notch-1 on glia induces morphological differentiation. Human DNER ECD is 89% aa identical to mouse DNER ECD.

Long Name
Delta/Notch-like EGF Repeat-containing Transmembrane
Entrez Gene IDs
92737 (Human); 227325 (Mouse)
Alternate Names
bet; delta and Notch-like epidermal growth factor-related receptor; delta/notch-like EGF repeat containing; delta-notch-like EGF repeat-containing transmembrane; DNER; H_NH0150O02.1; UNQ26; WUGSC:H_NH0150O02.1

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Citation for Human DNER Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Biological and molecular heterogeneity of breast cancers correlates with their cancer stem cell content.
    Authors: Pece S, Tosoni D, Confalonieri S, Mazzarol G, Vecchi M, Ronzoni S, Bernard L, Viale G, Pelicci PG, Di Fiore PP
    Cell, 2010-01-08;140(1):62-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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