Human DOK3 Antibody

Catalog #: MAB4795 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4795
MAB4795-SP

Detection of Human DOK3 by Western Blot.

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Human DOK3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human DOK3 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 642316

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human DOK3
Gly181-Lys271
Accession # Q7L591

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human DOK3 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 0.25 µg/mL of Mouse Anti-Human DOK3 Monoclonal Antibody (Catalog # MAB4795) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DOK3 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DOK3

DOK3 (downstream of kinase 3) is a 53 kDa (predicted) member of the DOK family of cytoplasmic adaptor proteins and is expressed in B cells and macrophages. Human DOK3 cDNA encodes 496 amino acids (aa) including one pleckstrin homology region (aa 63‑179) and an IRS-type phosphotyrosine binding domain (PTB) (aa 213‑317). Alternate splicing generates 330, 228 and 216 aa isoforms with N-terminal and C-terminal truncations and substitutions. Human DOK3 shares approximately 80% aa sequence identity with mouse and rat DOK3 within aa 181‑271, which are common to all four isoforms. Phosphorylation of tyrosine residues in DOK3 regulates its interaction with SH2 and SH3 containing proteins, leading to inhibition of signaling from immunoreceptors.

Long Name

Downstream of Tyrosine Kinase 3

Entrez Gene IDs

79930 (Human); 27261 (Mouse); 306760 (Rat)

Alternate Names

docking protein 3; DOK3; DOKL; Dok-like protein; Downstream of tyrosine kinase 3; FLJ22570; FLJ39939