Human Dopamine D5 R/DRD5 PE-conjugated Antibody

Catalog #: FAB82861P 1 Review Datasheet / COA / SDS

Discontinued Product

FAB82861P has been discontinued.
View all Dopamine D5R/DRD5 products.
Detection of Dopamine D5 R/DRD5 in HEK293 Human Cell Line Transfected with Human Dopamine D5 R/DRD5 and eGFP by Flow Cytometry.
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Human Dopamine D5 R/DRD5 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects HEK293 human embryonic kidney cell line transfected with human Dopamine D5 R/DRD5 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
Source
Monoclonal Mouse IgG2A Clone # 889022
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Dopamine D5 R/DRD5
Met1-His477
Accession # P21918
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Dopamine D5 R/DRD5 antibody in HEK293 Human Cell Line Transfected with Human Dopamine D5 R/DRD5 and eGFP antibody by Flow Cytometry. View Larger

Detection of Dopamine D5 R/DRD5 in HEK293 Human Cell Line Transfected with Human Dopamine D5 R/DRD5 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human Dopamine D5 R/DRD5 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Dopamine D5 R/DRD5 PE-conjugated Monoclonal Antibody (Catalog # FAB82861P). Quadrant markers were set based on control antibody staining (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Dopamine D5R/DRD5

DRD5, also known as Dopamine D5 Receptor has a higher affinity for neurotransmitter dopamine than DRD1 (1). DRD5 is expressed in neurons in many human brain regions, including cortex regions, hippocampus, choroid plexus, and brainstem (2). Polymorphisms in the DRD5 gene have been associated with Attention Deficit Hyperactivity Disorder (ADHD) (3), schizopherenia (4) and nicotine dependence (5). Dopamine receptors undergo endocytosis upon interaction with receptor agonists and by activation of protein kinase C (PKC) (6).

References
  1. Sunahra, R. et al. (1991) Nature. 350:614.
  2. Weinshank, R. et al. (1991) J Biol Chem. 266:22427.
  3. Squassina, A. et al.  (2008) Neurosci Lett. Feb 13.
  4. Golimbet, V. et al.  (2008) Bull Exp Biol Med. Jan.
  5. Wei, J. et al.  (2012) Addict Behav. 37:622.
  6. Thompson, D. et al. (2011) Traffic. 12:644.
Long Name
Dopamine D5 Receptor
Entrez Gene IDs
1816 (Human); 13492 (Mouse); 25195 (Rat)
Alternate Names
D(1B) dopamine receptor; D(5) dopamine receptor; D1beta dopamine receptor; DBDR; Dopamine D5 R; Dopamine D5 receptor; Dopamine D5R; dopamine receptor D1B; dopamine receptor D5; DRD1B; DRD1BDBDR; DRD1L2; DRD1L2MGC10601; DRD5

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Human Dopamine D5 R/DRD5 PE-conjugated Antibody
By Susan Sheng on 06/20/2017
Application: Flow Sample Tested: EOL-1 human acute myeloid (eosinophilic) leukemia cell line,IMR-32 human neuroblastoma cell line,KG-1 human acute myelogenous leukemia cell line,MV4-11 Species: Human