Human Dopamine D5R/DRD5 Antibody

Catalog #: MAB8286 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8286
MAB8286-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Dopamine D5R/DRD5 Antibody Summary

Species Reactivity

Human

Specificity

Detects HEK293 cells transfected with human Dopamine D5 R/DRD5 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.

Source

Monoclonal Mouse IgG₁ Clone # 889040

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Dopamine D5 R/DRD5
Accession # P21918

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Dopamine D5 R/DRD5 in Human Brain. Dopamine D5 R/DRD5 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human Dopamine D5 R/DRD5 Monoclonal Antibody (Catalog # MAB8286) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dopamine D5R/DRD5

DRD5, also known as Dopamine D5 Receptor has a higher affinity for neurotransmitter dopamine than DRD1 (1). DRD5 is expressed in neurons in many human brain regions, including cortex regions, hippocampus, choroid plexus, and brainstem (2). Polymorphisms in the DRD5 gene have been associated with Attention Deficit Hyperactivity Disorder (ADHD) (3), schizopherenia (4) and nicotine dependence (5). Dopamine receptors undergo endocytosis upon interaction with receptor agonists and by activation of protein kinase C (PKC) (6).

References

Sunahra, R. et al. (1991) Nature. 350:614.
Weinshank, R. et al. (1991) J Biol Chem. 266:22427.
Squassina, A. et al. (2008) Neurosci Lett. Feb 13.
Golimbet, V. et al. (2008) Bull Exp Biol Med. Jan.
Wei, J. et al. (2012) Addict Behav. 37:622.
Thompson, D. et al. (2011) Traffic. 12:644.

Long Name

Dopamine D5 Receptor

Entrez Gene IDs

1816 (Human); 13492 (Mouse); 25195 (Rat)

Alternate Names

D(1B) dopamine receptor; D(5) dopamine receptor; D1beta dopamine receptor; DBDR; Dopamine D5 R; Dopamine D5 receptor; Dopamine D5R; dopamine receptor D1B; dopamine receptor D5; DRD1B; DRD1BDBDR; DRD1L2; DRD1L2MGC10601; DRD5