Human DPCR-1 Antibody

Catalog #: MAB33471 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB33471-100
MAB33471-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DPCR-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human DPCR-1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 986124

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human DPCR-1 synthetic peptide
Accession # Q3MIW9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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DPCR‑1 in HT‑29 Human Cell Line. DPCR-1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line (positive control) and not U-87 MG human glioblastoma/astrocytoma cell line (negative control) using Mouse Anti-Human DPCR-1 Monoclonal Antibody (Catalog # MAB33471) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPCR-1

DPCR-1 (Diffuse Panbronchiolitis Critical Region 1) is encoded by a gene that maps to the chromosomal diffuse panbronchiolitis critical region. DPCR-1 is associated with susceptibility to this bronchiolar disease. It has homology with the mucin-like domain of zonadhesin. DPCR-1 is predominantly expressed in lung, kidney, and testis.

Long Name

Diffuse Panbronchiolitis Critical Region 1

Entrez Gene IDs

135656 (Human); 268949 (Mouse); 406199 (Rat)

Alternate Names

bCX105N19.6; C6orf37; diffuse panbronchiolitis critical region 1; DPCR1; DPCR-1; MGC126710; MGC126712; PBLT; PBLTdiffuse panbronchiolitis critical region 1 protein