Human DPPA5/ESG1 Antibody

Catalog # Availability Size / Price Qty
MAB3125
MAB3125-SP
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Human DPPA5/ESG1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DPPA5/ESG1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human DPPA4 or recombinant mouse DPPA5.
Source
Monoclonal Mouse IgG1 Clone # 319606
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human DPPA5/ESG1
Met1-Lys116
Accession # A6NC42
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human DPPA5/ESG1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPPA5/ESG1

Human DPPA5 (Developmental Pluripotency Associated 5), also called ESG1 (embryonal stem cell-specific 1) is expressed in embryonic germ cells and embryonic stem cells and is a potential marker for pluripotent stem cells. Human and mouse DPPA5 share 63% amino acid sequence identity.

Long Name
Developmental Pluripotency Associated 5
Entrez Gene IDs
340168 (Human); 434423 (Mouse); 301101 (Rat)
Alternate Names
developmental pluripotency associated 5; developmental pluripotency-associated 5 protein; DPPA5; embryonal stem cell specific gene 1; Embryonal stem cell-specific gene 1 protein; ESG1; ESG1ESG-1; hDPPA5

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