Home / DR6/TNFRSF21 / Human DR6/TNFRSF21 Antibody

Human DR6/TNFRSF21 Antibody

Catalog #: AF144
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF144
AF144-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DR6/TNFRSF21 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DR6 in ELISAs and Western blots. In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant human (rh) OPG and rhTNF RII is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DR6
Gln42-Leu350
Accession # O75509
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human DR6/TNFRSF21 Fc Chimera (Catalog # 144-DR)

Human DR6/TNFRSF21 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human DR6/TNFRSF21 Biotinylated Antibody (Catalog # BAF144)

Standard: Recombinant Human DR6/TNFRSF21 Fc Chimera Protein, CF (Catalog # 144-DR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DR6/TNFRSF21

Death Receptor 6 (DR6), also known as TNFRSF21 and CD358, is a type I transmembrane protein in the TNF receptor superfamily (1). Human DR6 consists of a 308 amino acid (aa) extracellular domain (ECD) with four cysteine‑rich motifs, a 21 aa transmembrane segment, and a 285 aa palmitylated cytoplasmic region that contains one death domain (2, 3). Within the ECD, human and mouse DR6 share 82% aa sequence identity. DR6 is expressed as an approximately 110 kDa molecule that carries extensive N‑linked and O‑linked glycosylation in its extracellular region (3, 4). Among hematopoietic cells, DR6 is expressed on monocytes, resting CD4+ T cells, and pro‑, pre‑, and naïve B cells (5 ‑ 7). DR6 knockout mice exhibit a Th2‑biased immune response characterized by exaggerated Th2 and B cell responsiveness in combination with reduced Th1 cell responsiveness and inflammatory leukocyte infiltration (6 ‑ 9). DR6 knockout mice are resistant to induced airway inflammation and experimental autoimmune encephalitis but more susceptible to severe graft versus host disease (9 ‑ 11). DR6 is also expressed on developing neurons where it can bind a shed 35 kDa N‑terminal fragment of APP or a fragment of APLP2 (12, 13). This APP fragment is generated following deprivation of neurotrophic factors, and its binding to DR6 triggers DR6‑mediated axonal pruning (12). DR6 is constitutively expressed on some prostate cancer cells and can be induced by TNF‑ alpha on others (3, 4).

References
  1. Benschop, R. et al. (2009) Adv. Exp. Med. Biol. 647:186.
  2. Pan, G. et al. (1998) FEBS Lett. 431:351.
  3. Klima, M. et al. (2009) Biochim. Biophys. Acta 1793:1579.
  4. Kasof, G.M. et al. (2001) Oncogene 20:7965.
  5. Matesanz-Isabel, J. et al. (2011) Immunol. Lett. 134:104.
  6. Schmidt, C.S. et al. (2003) J. Exp. Med. 197:51.
  7. Liu, J. et al. (2001) Immunity 15:23.
  8. Zhao, H. et al. (2001) J. Exp. Med. 194:1441.
  9. Venkataraman, C. et al. (2006) Immunol. Lett. 106:42.
  10. Schmidt, C.S. et al. (2005) J. Immunol. 175:2286.
  11. Liu, J. et al. (2002) J. Immunol. 169:3993.
  12. Nikolaev, A. et al. (2009) Nature 457:981.
  13. Kuester, M. et al. (2011) J. Mol. Biol. 409:189.
Long Name
Death Receptor 6
Entrez Gene IDs
27242 (Human); 94185 (Mouse); 316256 (Rat)
Alternate Names
BM-018; CD358 antigen; CD358; Death receptor 6; DR6; DR6TNFR-related death receptor 6; MGC31965; TNFRSF21; tumor necrosis factor receptor superfamily member 21; tumor necrosis factor receptor superfamily, member 21

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human DR6/TNFRSF21 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. MicroRNA-195 prevents dendritic degeneration and neuron death in rats following chronic brain hypoperfusion
    Authors: X Chen, XM Jiang, LJ Zhao, LL Sun, ML Yan, Y Tian, S Zhang, MJ Duan, HM Zhao, WR Li, YY Hao, LB Wang, QJ Xiong, J Ai
    Cell Death Dis, 2017-06-01;8(6):e2850.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Sensitization of imatinib-resistant CML cells to TRAIL-induced apoptosis is mediated through down-regulation of Bcr-Abl as well as c-FLIP.
    Authors: Park SJ, Kim MJ, Kim HB, Kang CD, Kim SH
    Biochem. J., 2009-04-28;420(1):73-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human DR6/TNFRSF21 Antibody

There are currently no reviews for this product. Be the first to review Human DR6/TNFRSF21 Antibody and earn rewards!

Have you used Human DR6/TNFRSF21 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review