Human DSCAM-L1 Antibody

Catalog #: AF3315
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3315
AF3315-SP

Detection of DSCAM‑L1 in A172 Human Cell Line by Flow Cytometry.

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All DSCAM-L1 Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DSCAM-L1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human DSCAM-L1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human DSCAM is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DSCAM-L1
Glu19-Lys1591
Accession # Q8TD84

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human DSCAM-L1

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of DSCAM‑L1 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Goat Anti-Human DSCAM-L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3315, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DSCAM-L1

DSCAM-L1 is a 250 kDa type I transmembrane Ig superfamily glycoprotein that is predominantly expressed in Purkinje cells and neurons of the cerebral cortex and olfactory bulb.DSCAM-L1 mediates cell adhesion by cation-independent homophilic interactions. It may function in the formation and maintenance of neural networks and play a role in neural disorders such as Gilles de la Tourette and Jacobsen syndromes. Human DSCAM-L1 contains ten Ig-like C2-type domains and six fibronectin type-III domains. An alternately spliced isoform is generated by deletion of 211 amino acids near the N-terminal. Within the extracellular domain, human DSCAM-L1 shares 98% amino acid sequence identity with mouse and rat DSCAM-L1.

Long Name

Down Syndrome Cell Adhesion Molecule Like 1

Entrez Gene IDs

57453 (Human); 114873 (Mouse); 315615 (Rat)

Alternate Names

Down syndrome cell adhesion molecule like 1; Down syndrome cell adhesion molecule-like protein 1; Downs syndrome cell adhesion molecule like 1; DSCAM2; DSCAML1; DSCAM-L1; KIAA1132Down syndrome cell adhesion molecule 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human DSCAM-L1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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