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Human E-Selectin/CD62E Antibody

Catalog #: BBA26
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BBA26-200
Detection of E-Selectin/CD62E in HUVECs by Flow Cytometry.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human E-Selectin/CD62E Antibody Summary

Species Reactivity
Human
Specificity
This antibody binds to CHO cells transfected with human E-Selectin. It does not bind to COS cells transfected with human PECAM‑1, P‑Selectin, L‑Selectin, VCAM‑1, or ICAM‑1.
Source
Monoclonal Mouse IgG1 Clone # BBIG-E1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
HUVEC human umbilical vein endothelial cells treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Immunoprecipitation
Pigott, R. et al. (1991) J. Immunol. 147:130.
 
Adhesion Blockade
The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human E‑Selectin (Catalog # ADP1, 2 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Immunofluorescence

Pigott, R. et al. (1991) J. Immunol. 147:130.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of E-Selectin/CD62E in HUVECs by Flow Cytometry. Human umbilical cord endothelial cells (HUVECs) were cultured for 6 hours in the presence of 25 ng/mL of rhTNF-alpha (210-TA, filled histogram) or rested (open histogram) and stained with Mouse anti-human E-selectin/CD62E (BBA26) followed by Goat anti-Mouse APC-conjugated secondary antibody (F0101B). Gates were set based on isotype control (MAB002, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

Immunocytochemistry E-Selectin/CD62E antibody in HUVECs by Immunocytochemistry (ICC). View Larger

E‑Selectin/CD62E in HUVECs. E-Selectin/CD62E was detected in immersion fixed HUVEC human umbilical vein endothelial cells stimulated with Recombinant Human TNF-alpha (Catalog # 210-TA) using Mouse Anti-Human E-Selectin/CD62E Monoclonal Antibody (Catalog # BBA26) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E-Selectin/CD62E

E-Selectin, also known as ELAM-1, is a transmembrane glycoprotein that is transiently expressed on activated vascular endothelial cells. E-Selectin binds to sialyated, fucosylated molecules on neutrophils, monocytes, and a subset of memory T cells.

Entrez Gene IDs
6401 (Human); 20339 (Mouse); 25544 (Rat); 102143564 (Cynomolgus Monkey)
Alternate Names
CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E

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Citations for Human E-Selectin/CD62E Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Cargo-free particles divert neutrophil-platelet aggregates to reduce thromboinflammation
    Authors: AL Banka, MV Guevara, ER Brannon, NQ Nguyen, S Song, G Cady, DJ Pinsky, KE Uhrich, R Adili, M Holinstat, O Eniola-Ade
    Nature Communications, 2023-04-28;14(1):2462.
  2. Zika Virus Infection Promotes Local Inflammation, Cell Adhesion Molecule Upregulation, and Leukocyte Recruitment at the Blood-Brain Barrier
    Authors: M Clé, C Desmetz, J Barthelemy, MF Martin, O Constant, G Maarifi, V Foulongne, K Bolloré, Y Glasson, F De Bock, M Blaquiere, L Dehouck, N Pirot, E Tuaillon, S Nisole, F Najioullah, P Van de Per, A Cabié, N Marchi, F Gosselet, Y Simonin, S Salinas
    MBio, 2020-08-04;11(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Recombinant Human Vimentin Binds to P-Selectin and Blocks Neutrophil Capture and Rolling on Platelets and Endothelium
    Authors: FW Lam, Q Da, B Guillory, MA Cruz
    J. Immunol., 2018-01-15;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Blocking
  4. A Glycovariant of Human CD44 is Characteristically Expressed on Human Mesenchymal Stem Cells
    Stem Cells, 2017-02-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Annexin A8 controls leukocyte recruitment to activated endothelial cells via cell surface delivery of CD63.
    Authors: Poeter M, Brandherm I, Rossaint J, Rosso G, Shahin V, Skryabin B, Zarbock A, Gerke V, Rescher U
    Nat Commun, 2014-04-28;5(0):3738.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  6. Leukemia Mediated Endothelial Cell Activation Modulates Leukemia Cell Susceptibility to Chemotherapy through a Positive Feedback Loop Mechanism.
    Authors: Pezeshkian, Bahareh, Donnelly, Christop, Tamburo, Kelley, Geddes, Timothy, Madlambayan, Gerard J
    PLoS ONE, 2013-04-01;8(4):e60823.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  7. The adaptor molecule Lnk negatively regulates tumor necrosis factor-alpha-dependent VCAM-1 expression in endothelial cells through inhibition of the ERK1 and -2 pathways.
    Authors: Fitau J, Boulday G, Coulon F, Quillard T, Charreau B
    J. Biol. Chem., 2006-04-27;281(29):20148-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Ligation of ICAM-1 on endothelial cells leads to expression of VCAM-1 via a nuclear factor-kappaB-independent mechanism.
    Authors: Lawson C, Ainsworth M, Yacoub M, Rose M
    J. Immunol., 1999-03-01;162(5):2990-6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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