Human EAAT1/GLAST-1 Antibody

Catalog #: MAB6048 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6048-100
MAB6048-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EAAT1/GLAST-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human EAAT1/GLAST-1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 482420

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human EAAT1/GLAST‑1
His146-Ser237
Accession # P43003

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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EAAT1/GLAST-1 in Human Brain Tissue. EAAT1/GLAST-1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) tissue using Mouse Anti-Human EAAT1/GLAST-1 Monoclonal Antibody (Catalog # MAB6048) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal puncta in molecular layer. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EAAT1/GLAST-1

GLAST-1 (Sodium dependent GLu/ASp Transporter 1; also SLC1A3 and EAAT1) is a 60‑62 kDa member of the SDF symporter family of molecules. It is expressed by glia, fibroblasts, and select neuron cell types such as hippocampal neurons. EAAT1 is known to transport L‑glutamate into glia, thus removing glutamate from synaptic areas where it either acts too long, or becomes toxic at elevated concentration. Its action is dependent on its ability to cotransport sodium. Human EAAT1 is a 542 amino acid (aa), 8-transmembrane variably glycosylated protein that contains N- and C-terminal cytoplasmic domains. Homodimers are reported to exist, but only when EAAT1 shows glycosylation. There are at least two potential isoform variants. One shows an Arg substitution for aa 430‑475, while a second shows a four aa substitution for aa 62‑542. Over an extracellular loop that encompasses aa 146‑237, human EAAT1 shares 92% aa identity with mouse EAAT1.

Long Name

Excitatory Amino Acid Transporter 1/Sodium-dependent Glu/Asp Transporter 1

Entrez Gene IDs

6507 (Human); 20512 (Mouse); 29483 (Rat)

Alternate Names

EA6; EA6FLJ25094; EAAT1; EAAT1GLAST-1; excitatory amino acid transporter 1; GLAST1; GLAST-1; GLASTGLAST1; SLC1A3; Sodium-dependent glutamate/aspartate transporter 1; solute carrier family 1 (glial high affinity glutamate transporter), member 3; Solute carrier family 1 member 3