Human EDA-A2/Ectodysplasin A2 Antibody

Catalog # Availability Size / Price Qty
MAB922
MAB922-SP
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Human EDA-A2/Ectodysplasin A2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human EDA‑A2/Ectodysplasin A2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross‑reactivity with recombinant human (rh) EDA-A1 is observed, approximately 10% cross‑reactivity with recombinant mouse EDA-A1 is observed, and no cross‑reactivity with rhAPRIL, rhBAFF, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhOX40 Ligand, rhTNF-alpha, rhTRAIL, rhTRANCE, rhTWEAK, and rhVEGI is observed.
Source
Monoclonal Mouse IgG1 Clone # 174813
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EDA‑A2/Ectodysplasin A2
Ala179-Ser389
Accession # NP_001005609
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human EDA‑A2/Ectodysplasin A2 (Catalog # 922-ED)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EDA-A2/Ectodysplasin A2

EDA-A2, a product of the EDA gene (also called Tabby), is a type II transmembrane protein that is a member of the TNF Superfamily (TNFSF). Human EDA-A2 is a 389 amino acid (aa) protein with a predicted N-terminal 39 aa cytoplasmic domain, a 22 aa transmembrane domain and a C-terminal 328 aa extracellular domain. The extracellular domains of human and mouse EDA-A2 share approximately 94% identity. Within the TNFSF, EDA-A2 shares the highest homology with EDA-A1, the other product of the EDA gene. EDA-A2 and EDA-A1 are splice variants of EDA which differ by only two amino acids. EDA-A1 contains two additional amino acids, Glu308 and Val309. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds EDAR, a member of the TNF Receptor Superfamily (TNFRSF), whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF family member with high homology to EDAR. Mutations in EDA-A1, EDA-A2, EDAR and XEDAR have been associated with hypohidrotic ectodermal dysplasia (HED). HED is characterized by abnormalities in hair, teeth and eccrine sweat gland morphogenesis. HED was initially found to associate with two gene loci, tabby and downless. Tabby was later identified as the gene for EDA and downless as the autosomal EDAR gene.

References
  1. Bayes, M. et al. (1998) Hum. Mol. Genet. 7:1661.
  2. Kere, J. et al. (1996) Nat. Genet. 13:409.
  3. Monreal, A.W. et al. (1998) Am. J. Hum. Genet. 63:380.
  4. Headon, D.J. and P.A. Overbeek (1999) Nat. Genet. 22(4):370.
  5. Kumar, A. et al. (2000) J. Biol. Chem. 276(4):2668.
  6. Monreal, A.W. et al. (1999) Nat. Genet. 22(4):366.
  7. Srivastava, A.K. et al. (1997) Proc. Natl. Acad. Sci. USA 94(24):13069.
  8. Yan, M. et al. (2000) Science 290(5491):523.
Entrez Gene IDs
1896 (Human); 13607 (Mouse)
Alternate Names
ECTD1;Ectodysplasin-A;ED1;ED1-A1;ED1-A2;EDA1;EDA2;EDA-A1;EDA-A2;HED;HED1;ODT1;STHAGX1;TNLG7C;XHED;XLHED; EDAA2; EDA-A2

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