Human EG-VEGF/PK1 Antibody

Catalog #: MAB12091 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB12091
MAB12091-SP

All EG-VEGF/PK1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EG-VEGF/PK1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human EG‑VEGF/PK1 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 188608

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human EG‑VEGF/PK1
Ala20-Phe105
Accession # P58294

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Human EG-VEGF/PK1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human EG‑VEGF/PK1 Biotinylated Antibody (Catalog # BAF1209)

Standard: Recombinant Human EG-VEGF/PK1 Protein (Catalog # 1209-EV)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EG-VEGF/PK1

Endocrine gland-derived vascular endothelial growth factor (EG-VEGF), also called prokineticin 1 (PK1), is a member of the prokineticin family of secreted proteins that share a common structural motif containing ten conserved cysteine residues that form five pairs of disulfide bonds (1, 2). Members of this family include the mammalian EG-VEGF/PK1 and PK2, as well as the venom protein A (VPRA) from the venom of black mamba snake and the frog Bombina variegata, Bv8 (1). Human EG-VEGF precursor is a 105 amino acid (aa) residue protein with a 19 aa signal peptide that is cleaved to yield a 86 aa mature protein (1, 2). EG-VEGF is expressed in multiple tissues including the gastrointestinal (GI) tract and steroidogenic glands (testis, ovary, placenta and adrenal glands). EG-VEGF has been shown to potently stimulate the contraction of GI smooth muscle. In addition, EG-VEGF is a tissue-specific angiogenic factor that exhibits biological activities similar to that of VEGF on select cells. It induces the proliferation, migration, and fenestration in cultured endocrine gland-derived capillary endothelial cells. EG-VEGF binds to and activates two closely related G protein-coupled receptors, EG-VEGF/PK1-R1 and EG-VEGF/PK2-R2 (3, 4). Activation of the receptors leads to stimulation of phosphoinositide turnover and activation of p44/p42 MAP kinase signaling pathways.

References

Li, M. et al. (2001) Mol. Pharmacol. 59:692.
LeCouter, J. et al. (2001) Nature 412:877.
Lin, D. et al. (2002) J. Biol. Chem. 277:19276.
Masuda, Y. et al. (2002) Biochem. Biophys. Res. Commun. 293:396.

Long Name

Endocrine Gland-derived Vascular Endothelial Growth Factor

Entrez Gene IDs

84432 (Human); 246691 (Mouse); 192205 (Rat)

Alternate Names

EGVEGF; EG-VEGF; EGVEGFblack mamba toxin-related protein; Endocrine-gland-derived vascular endothelial growth factor; Mambakine; PK1mambakine; PRK1prokineticin-1; PROK1; Prokineticin 1