Human EGR1 Antibody

Catalog #: MAB28181 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB28181-100
MAB28181-SP

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Human EGR1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human EGR1 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 406910

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human EGR1
Gln282-Ser433
Accession # P18146

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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EGR1 in Human Hodgkin's Lymphoma. EGR1 was detected in immersion fixed paraffin-embedded sections of human Hodgkin's Lymphoma using Rat Anti-Human EGR1 Monoclonal Antibody (Catalog # MAB28181) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry

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EGR1 in Human Adenocarcinoma Tissue. EGR1 was detected in immersion fixed paraffin-embedded sections of human adenocarcinoma tissue using Rat Anti-Human EGR1 Monoclonal Antibody (Catalog # MAB28181) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGR1

Early Growth Response 1, (EGR1) belongs to the EGR family of zinc finger transcription factors and regulates the expression of several tumor suppressors. Its expression is induced by diverse signals that initiate growth and differentiation. EGR1 suppresses or activates cell growth depending on the tumor type. Within the region that includes its three zinc finger domains, human EGR1 shares 99% aa sequence identity with mouse and rat EGR1.

Long Name

Early Growth Response 1

Entrez Gene IDs

1958 (Human)

Alternate Names

AT225Transcription factor ETR103; early growth response 1; early growth response protein 1; EGR1; EGR-1; G0S30; KROX24; KROX-24; Nerve growth factor-induced protein A; NGFIA; NGFI-ATranscription factor Zif268; TIS8; ZIF-268; Zinc finger protein Krox-24; ZNF225; ZNF225Zinc finger protein 225