Human eIF4B Antibody

Name: Human eIF4B Antibody
Brand: R&D Systems
SKU: AF3800
Rating: 5 (1 reviews)

Catalog #: AF3800 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3800
AF3800-SP

Detection of Human eIF4B by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human eIF4B Antibody Summary

Species Reactivity

Human

Specificity

Detects human and mouse eIF4B in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human eIF4B
Met1-Arg200
Accession # P23588

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human eIF4B by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human eIF4B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3800) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eIF4B at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: eIF4B

eIF4B (eukaryotic translation initiation factor 4B),an RNA binding protein, is essential for the binding of mRNA to the 43S pre-initiation complex, which consists of the 40S ribosomal subunit bound to a ternary complex of eIF2, GTP, and Met-tRNA stabilized by eIF3. An arginine rich motif (ARM) in the carboxy-terminus of eIF4B binds RNA non-specifically, while a canonical RNA motif (RMM) near the amino terminus binds specifically to 18S rRNA. The simultaneous binding of specific and nonspecific RNA may serve to facilitate the binding of the 40S subunit to the mRNA by serving as a bridge between the 18S rRNA and the mRNA. Besides its binding activity, eIF4B in conjunction with eIF4F, a heterotrimeric protein, stimulates the ATPase and RNA helicase activity of eIF4E.

Long Name

Eukaryotic Translation Initiation Factor 4B

Entrez Gene IDs

1975 (Human); 75705 (Mouse); 300253 (Rat)

Alternate Names

Eif4a2; eIF4B; EIF-4B; eukaryotic translation initiation factor 4B; PRO1843