Human EMR2 Antibody

Catalog #: MAB48941 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB48941-100
MAB48941-SP

Detection of EMR2 in HEK293 Human Cell Line Transfected with Human EMR2 and eGFP by Flow Cytometry

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Human EMR2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human EMR2 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 494014

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human EMR2
Gln24-Asn823
Accession # AAI27006

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 Human Cell Line Transfected with Human EMR2 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of EMR2 in HEK293 Human Cell Line Transfected with Human EMR2 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with (A) human EMR2 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human EMR2 Monoclonal Antibody (Catalog # MAB48941) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB0041). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EMR2

EMR2 (EGF-like module-containing mucin-like receptor 2; designated CD312) is a glycoprotein belonging to the EGF-TM7 family of adhesion-type class B 7‑transmembrane (TM) receptors. EGF-like sequences within long extracellular N-termini, and a GPS (G-protein proteolytic site) domain are characteristic of this family, which is mainly expressed on cells of the immune system (1, 2). The human EMR2 cDNA encodes an 823 amino acid (aa) protein with five EGF-like domains within the first 250 aa, followed by a mucin-like stalk, a GPS domain (aa 479‑530) and a 7‑TM sequence (aa 531‑785). The GPS domain is the site of autocatalytic cleavage, forming two cleaved portions that remain non-covalently attached as a heterodimer (1, 3). Of the first 290 aa of human EMR2, 284 aa (97%) are identical with family member CD97, likely due to gene duplication (2). The portion of human EMR2 N-terminal to the GPS domain (aa 1‑478) shares 64%, 59%, 48% and 45% aa identity with corresponding regions of canine EMR2, equine EMR2, mouse CD97 and rat CD97, respectively. Alternate splicing of EMR2 creates isoforms that contain 2‑5 EGF-like domains. Only the 5-EGF form contains EGF4, which is necessary for calcium-dependent binding of the EMR2/CD97 ligand, chondroitin sulfate (CS) (2, 4‑6). None of the isoforms engage the CD97 ligand, CD55 (DAF). EMR2 is restricted to myeloid cells (1, 2). EMR2 expression increases as monocytes differentiate into macrophages, and decreases with differentiation into dendritic cells (5). Activation increases neutrophil EMR2 expression (5). EMR2 localizes to the leading edge of migrating neutrophils and plays an important role in migration, adhesion and superoxide production (7). It is also thought to facilitate specific interaction of myeloid cells with peripheral B lymphocytes which express CS (6).

References

Kwakkenbos, M.J. et al. (2004) Immunogenetics 55:655.
Lin, H.-H. et al. (2000) Genomics 67:188.
Lin, H.-H. et al. (2004) J. Biol. Chem. 279:31823.
Stacey, M. et al. (2003) Blood 102:2916.
Chang G.-W. et al. (2007) Biochem. Biophys. Res. Commun. 353:133.
Kwakkenbos, M.J. et al. (2005) J. Leukoc. Biol. 77:112.
Yona, S. et al. (2008) FASEB J. 22:741.

Long Name

EGF-like Module Containing, Mucin-like, Hormone Receptor-like 2

Entrez Gene IDs

30817 (Human)

Alternate Names

CD312; DKFZp781B135; egf-like module containing, mucin-like, hormone receptor-like 2; egf-like module containing, mucin-like, hormone receptor-like sequence 2; EGF-like module receptor 2; EGF-like module-containing mucin-like hormone receptor-like 2; EMR2