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Human Endoglycan/PODXL2 Antibody

Catalog #: MAB1524
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1524
MAB1524-SP
Endoglycan/PODXL2 in HeLa Human Cell Line.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Endoglycan/PODXL2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Endoglycan/PODXL2 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 211816
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Endoglycan/PODXL2
Gly33-Thr500
Accession # Q9NZ53
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
HUVEC human umbilical vein endothelial cells
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Endoglycan/PODXL2 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Endoglycan/PODXL2 in HeLa Human Cell Line. Endoglycan/PODXL2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Endoglycan/PODXL2 Monoclonal Antibody (Catalog # MAB1524) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endoglycan/PODXL2

Endoglycan, also named Podocalyxin-like 2 protein, is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. It is expressed in hematopoietic precursors, endothelial cells, smooth muscle cells and leukocyte subpopulations. Endoglycan is a potential marker for hematopoietic progenitors. It also functions as an L-Selectin ligand and may be involved in the inhibition of stem cell differentiation and leukocyte-endothelial adhesion (1, 2).

References
  1. Sassetti, C. et al. (2000) J. Biol. Chem. 275:9001.
  2. Fieger, C.B. et al. (2003) J. Biol. Chem. 278:27390.
Entrez Gene IDs
50512 (Human); 319655 (Mouse)
Alternate Names
Endoglycan; PODLX2; podocalyxin-like 2; podocalyxin-like protein 2; PODXL2

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Citations for Human Endoglycan/PODXL2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Identification of the SHREK family of proteins as broad-spectrum host antiviral factors
    Authors: D Dabbagh, S He, B Hetrick, L Chilin, A Andalibi, Y Wu
    bioRxiv : the preprint server for biology, 2021-02-03;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Capture, ICC
  2. Endoglycan, a member of the CD34 family of sialomucins, is a ligand for the vascular selectins.
    Authors: Kerr SC, Fieger CB, Snapp KR, Rosen SD
    J. Immunol., 2008-07-15;181(2):1480-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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