Human Endothelin-1 Antibody

Catalog #: MAB34402 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB34402-100
MAB34402-SP

Detection of Endothelin‑1 in Huvec (positive) and Jurkat (negative) cells.

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Human Endothelin-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Endothelin-1 in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1065402

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide containing EDN1.
Accession # P05305

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

Immersion fixed HUVEC human umbilical vein endothelial cells (positive) and Jurkat human acute T cell leukemia cells (negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of Endothelin‑1 in Huvec (positive) and Jurkat (negative) cells. Endothelin‑1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells (positive) and absent in Jurkat human acute T cell leukemia cells (negative) using Mouse Anti-Human Endothelin‑1 Monoclonal Antibody (Catalog # MAB34402) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endothelin-1

Endothelin-1, -2, and -3 are encoded by three separate genes as prepropolypeptides that are cleaved to yield inactive big endothelins. Big Endothelins are further cleaved to generate the 21 amino acid bioactive mature peptides. Endothelin-1 has potent vasoconstricting and angiogenic activity that is mediated through the 7TM receptors, ET-A and ET-B. Endothelin-1, -2, and -3 share identical amino acid sequences at their C-termini (residues 15‑21).

Entrez Gene IDs

1906 (Human); 13614 (Mouse); 24323 (Rat)

Alternate Names

ARCND3; EDN1; endothelin 1; Endothelin-1; ET1; HDLCQ7; PPET1; preproendothelin-1; QME

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