Human Endothelin-1 QuantiGlo ELISA Kit Summary
Product Summary
Recovery
The recovery of synthetic human Endothelin-1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 101 | 93-107 |
EDTA Plasma (n=4) | 94 | 88-99 |
Serum (n=4) | 95 | 87-103 |
Urine (n=4) | 98 | 92-105 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: Endothelin-1
Endothelin-1 (ET-1), a peptide of 21 amino acid (aa) residues, is a pleiotropic molecule best known for its action as a potent vasoconstrictor (1). Originally isolated from porcine aortic endothelial cells, ET-1 is one of a family of three proteins encoded by distinct genes that also includes Endothelin-2 (ET-2) and Endothelin-3 (ET-3) (2, 3). ET-2 and ET-3 differ from ET-1 by 2 and 6 amino acids, respectively (1, 2). All members of the Endothelin family contain two essential disulfide bridges and six conserved aa residues at the C-terminus. Human ET-1 is initially synthesized as a pre-pro-polypeptide of 212 amino acids (2, 4). It is proteolytically cleaved by a signal peptidase to produce pro-ET-1 and further processed by a Furin-like protease to yield a 38 aa peptide termed Big ET-1 (5, 6). Big ET-1 is then cleaved by the membrane-bound metalloprotease Endothelin-converting enzyme (ECE-1), producing the potent 21 aa mature form ET-1 (aa 1-21) (7, 8). Alternatively, ET-1 may exist in an active 31 aa form (ET-1 (aa 1-31)) following cleavage of Big ET-1 by chymase (9-12). The vascular endothelium is an abundant source of ET-1 (3, 13). It may also be expressed by leukocytes, smooth muscle cells, mesangial cells, cardiac myocytes, and astrocytes (14, 15). ET-1 can be induced in endothelial cells by many factors including mechanical stimulation, various hormones, and pro-inflammatory cytokines (16). Production is inhibited by nitric oxide (NO), Prostacyclin, and atrial natriuretic peptide (ANP) (17-19).
Citations for Human Endothelin-1 QuantiGlo ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Transpulmonary Plasma Endothelin-1 Arterial:Venous Ratio Differentiates Survivors from Non-Survivors in Critically Ill Patients with COVID-19-Induced Acute Respiratory Distress Syndrome
Authors: Vassiliou, AG;Roumpaki, A;Keskinidou, C;Athanasiou, N;Tsipilis, S;Jahaj, E;Vrettou, CS;Giannopoulou, V;Halioti, A;Ferentinos, G;Dimopoulou, I;Kotanidou, A;Langleben, D;Orfanos, SE;
International journal of molecular sciences
Species: Human
Sample Types: Plasma
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Effect of endothelin-1 on the blood pressure response to acute hypoxia and hyperoxia in healthy young men
Authors: Gonsalves, AM;Baker, SE;Jacob, DW;Harper, JL;Manrique-Acevedo, CM;Limberg, JK;
Physiological reports
Species: Human
Sample Types: Plasma
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Crucial Interactions between Altered Plasma Trace Elements and Fatty Acids Unbalance Ratio to Management of Systemic Arterial Hypertension in Diabetic Patients: Focus on Endothelial Dysfunction
Authors: Gouaref, I;Otmane, A;Makrelouf, M;Abderrhmane, SA;Haddam, AEM;Koceir, EA;
International journal of molecular sciences
Species: Human
Sample Types: Plasma
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Myocardial transcriptomic analysis of diabetic patients with aortic stenosis: key role for mitochondrial calcium signaling
Authors: Cherpaz, M;Meugnier, E;Seillier, G;Pozzi, M;Pierrard, R;Leboube, S;Farhat, F;Vola, M;Obadia, JF;Amaz, C;Chalabreysse, L;May, C;Chanon, S;Brun, C;Givre, L;Bidaux, G;Mewton, N;Derumeaux, G;Bergerot, C;Paillard, M;Thibault, H;
Cardiovascular diabetology
Species: Human
Sample Types: Serum
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The Regulation of Endothelin-1 in Pregnancies Complicated by Gestational Diabetes: Uncovering the Vascular Effects of Insulin
Authors: Fato, BR;Beard, S;Binder, NK;Pritchard, N;Kaitu'u-Lino, TJ;de Alwis, N;Hannan, NJ;
Biomedicines
Species: Human
Sample Types: Plasma
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Interplay of Race and Neighborhood Deprivation on Ambulatory Blood Pressure in Young Adults
Authors: Jeong, S;Linder, BA;Barnett, AM;Tharpe, MA;Hutchison, ZJ;Culver, MN;Sanchez, SO;Nichols, OI;Grosicki, GJ;Bunsawat, K;Nasci, VL;Gohar, EY;Fuller-Rowell, TE;Robinson, AT;
medRxiv : the preprint server for health sciences
Species: Human
Sample Types: Urine
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Vascular Function, Systemic Inflammation, and Coagulation Activation 18 Months after COVID-19 Infection: An Observational Cohort Study
Authors: LH Willems, LMC Jacobs, LA Groh, H Ten Cate, HMH Spronk, B Wilson-Sto, G Hannink, SMJ van Kuijk, C Ghossein-D, M Nagy, DHJ Thijssen, AS van Peters, MC Warlé
Journal of Clinical Medicine, 2023-02-10;12(4):.
Species: Human
Sample Types: Plasma
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Dietary sodium restriction prevents vascular endothelial growth factor inhibitor-induced hypertension
Authors: L van Doorn, WJ Visser, DCH van Dorst, KM Mirabito C, SLW Koolen, AVE de Mik, IM Garrelds, DM Bovée, EO de Hoop, S Bins, FALM Eskens, EJ Hoorn, AH Jan Danser, RHJ Mathijssen, J Versmissen
British Journal of Cancer, 2022-11-10;0(0):1-9.
Species: Human
Sample Types: Plasma
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Endothelial dysfunction and altered endothelial biomarkers in patients with post-COVID-19 syndrome and chronic fatigue syndrome (ME/CFS)
Authors: M Haffke, H Freitag, G Rudolf, M Seifert, W Doehner, N Scherbakov, L Hanitsch, K Wittke, S Bauer, F Konietschk, F Paul, J Bellmann-S, C Kedor, C Scheibenbo, F Sotzny
Journal of Translational Medicine, 2022-03-22;20(1):138.
Species: Human
Sample Types: Serum
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Association of Plasma Endothelin-1 with Blood Pressure Progression Among African Americans: The Jackson Heart Study
Authors: AD Kaze, X Gao, SK Musani, A Bidulescu, AG Bertoni, M Abdalla, JB Echouffo-T
American heart journal, 2022-01-02;0(0):.
Species: Human
Sample Types: Serum
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Serum Metabolomic Analysis of Coronary Heart Disease Patients with Stable Angina Pectoris Subtyped by Traditional Chinese Medicine Diagnostics Reveals Biomarkers Relevant to Personalized Treatments
Authors: N Guo, P Wang, J Yang, X Yang, M van der Vo, M Wildwater, J Wei, X Tang, M Wang, H Yang
Frontiers in Pharmacology, 2021-06-14;12(0):664320.
Species: Human
Sample Types: Serum
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Adaptive response triggered by the repeated SCUBA diving is reflected in cardiovascular, muscular, and immune biomarkers
Authors: M Žarak, A Perovi?, M Njire Brat, S Šupraha Go, J Dumi?
Physiological Reports, 2021-01-01;9(2):e14691.
Species: Human
Sample Types: Plasma
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EDN1-AS, A Novel Long Non-coding RNA Regulating Endothelin-1 in Human Proximal Tubule Cells
Authors: LG Douma, K Solocinski, SH Masten, DH Barral, SJ Barilovits, LA Jeffers, KD Alder, R Patel, CS Wingo, KD Brown, BD Cain, ML Gumz
Front Physiol, 2020-03-13;11(0):209.
Species: Human
Sample Types: Cell culture supernate
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FURIN Expression in Vascular Endothelial Cells Is Modulated by a Coronary Artery Disease-Associated Genetic Variant and Influences Monocyte Transendothelial Migration
Authors: X Yang, W Yang, DG McVey, G Zhao, J Hu, RN Poston, M Ren, K Willeit, S Coassin, J Willeit, TR Webb, NJ Samani, M Mayr, S Kiechl, S Ye
J Am Heart Assoc, 2020-02-11;9(4):e014333.
Species: Human
Sample Types: Cell Culture Supernates
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Clinical Significance of High-Mobility Group Box 1 Protein (HMGB1) and Nod-Like Receptor Protein 3 (NLRP3) in Patients with Ulcerative Colitis
Authors: Y Chen, D Wu, L Sun
Med. Sci. Monit., 2020-01-05;26(0):e919530.
Species: Human
Sample Types: Serum
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Plasma endothelin-1 levels are increased in atrial fibrillation patients with hyperthyroidism
Authors: F Mayyas, N Saadeh, K Al-Muqbel, DR Van Wagone
PLoS ONE, 2018-12-04;13(12):e0208206.
Species: Human
Sample Types: Plasma
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DKK1 and Kremen Expression Predicts the Osteoblastic Response to Bone Metastasis
Authors: KL Clines, GA Clines
Transl Oncol, 2018-05-15;11(4):873-882.
Species: Human
Sample Types: Cell Culture Supernates
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Intervention of integrative medicine treatment has impact on serum levels of ET-1, TNF-?, MLT in RA-CVD
Authors: M Chen, Z Li, Z Zhang, Y Du, Y Zhang, M Xu, C Sun
Saudi J Biol Sci, 2018-05-14;25(5):959-964.
Species: Human
Sample Types: Serum
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Acute Pressor Response to Psychosocial Stress Is Dependent on Endothelium-Derived Endothelin-1
Authors: BM Fox, BK Becker, AS Loria, KA Hyndman, C Jin, H Clark, R Johns, M Yanagisawa, DM Pollock, JS Pollock
J Am Heart Assoc, 2018-02-16;7(4):.
Species: Mouse
Sample Types: Plasma
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Impaired endothelial function in patients with cryptogenic stroke and patent foramen ovale is not affected by closure
Authors: M Lantz, K Kostulas, M Settergren, C Sjöstrand
J Interv Cardiol, 2017-04-25;0(0):.
Species: Human
Sample Types: Serum
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Endothelin receptor-specific control of endoplasmic reticulum stress and apoptosis in the kidney
Authors: C De Miguel, WC Hamrick, JL Hobbs, DM Pollock, PK Carmines, JS Pollock
Sci Rep, 2017-02-23;7(0):43152.
Species: Rat
Sample Types: Plasma
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Free radical scavenging decreases endothelin-1 excretion and glomerular albumin permeability during type 1 diabetes
Physiol Rep, 2016-12-01;4(24):.
Species: Rat
Sample Types: Urine
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Allergic Inflammation Leads to Neuropathic Pain via Glial Cell Activation
Authors: Jun-Ichi Kira
J. Neurosci., 2016-11-23;36(47):11929-11945.
Species: Human
Sample Types: Serum
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Effect of OSA on hypoxic and inflammatory markers during CPAP withdrawal: Further evidence from three randomized control trials
Authors: Malcolm Kohler
Respirology, 2016-11-18;0(0):.
Species: Human
Sample Types: Plasma
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Albuminuria Is Associated with Endothelial Dysfunction and Elevated Plasma Endothelin-1 in Sickle Cell Anemia
PLoS ONE, 2016-09-26;11(9):e0162652.
Species: Human
Sample Types: Plasma
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p66Shc regulates renal vascular tone in hypertension-induced nephropathy
Authors: Bradley Miller
J Clin Invest, 2016-06-06;0(0):.
Species: Rat
Sample Types: Tissue Culture Supernates
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Endothelin-1 Predicts Hemodynamically Assessed Pulmonary Arterial Hypertension in HIV Infection.
Authors: Parikh R, Ma Y, Scherzer R, Heringer A, Macgregor J, Martin J, Deeks S, Ganz P, Hsue P
PLoS ONE, 2016-01-11;11(1):e0146355.
Species: Human
Sample Types: Plasma
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Association of endothelin-1 and matrix metallopeptidase-9 with metabolic syndrome in middle-aged and older adults.
Authors: Yu A, Tam B, Yau W, Chan K, Yu S, Chung T, Siu P
Diabetol Metab Syndr, 2015-12-10;7(0):111.
Species: Human
Sample Types: Serum
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Optimum AT1 receptor-neprilysin inhibition has superior cardioprotective effects compared with AT1 receptor blockade alone in hypertensive rats.
Authors: Roksnoer L, van Veghel R, de Vries R, Garrelds I, Bhaggoe U, Friesema E, Leijten F, Poglitsch M, Domenig O, Clahsen-van Groningen M, Hoorn E, Jan Danser A, Batenburg W
Kidney Int, 2015-04-01;88(1):109-20.
Species: Rat
Sample Types: Plasma
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Chemotherapy-induced pulmonary hypertension: role of alkylating agents.
Authors: Ranchoux B, Gunther S, Quarck R, Chaumais M, Dorfmuller P, Antigny F, Dumas S, Raymond N, Lau E, Savale L, Jais X, Sitbon O, Simonneau G, Stenmark K, Cohen-Kaminsky S, Humbert M, Montani D, Perros F
Am J Pathol, 2014-12-11;185(2):356-71.
Species: Rabbit
Sample Types: Plasma
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The in vitro pharmacology of GS-5759, a novel bifunctional phosphodiesterase 4 inhibitor and long acting beta2-adrenoceptor agonist.
Authors: Tannheimer S, Sorensen E, Cui Z, Kim M, Patel L, Baker W, Phillips G, Wright C, Salmon M
J Pharmacol Exp Ther, 2014-02-10;349(1):85-93.
Species: Human
Sample Types: Cell Culture Supernates
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Aberrant chloride intracellular channel 4 expression contributes to endothelial dysfunction in pulmonary arterial hypertension.
Authors: Wojciak-Stothard B, Abdul-Salam V, Lao K, Tsang H, Irwin D, Lisk C, Loomis Z, Stenmark K, Edwards J, Yuspa S, Howard L, Edwards R, Rhodes C, Gibbs J, Wharton J, Zhao L, Wilkins M
Circulation, 2014-02-06;129(17):1770-80.
Species: Human
Sample Types: Cell Culture Supernates
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miR-218 regulates focal adhesion kinase-dependent TGFbeta signaling in fibroblasts.
Authors: Guo F, Carter D, Leask A
Mol Biol Cell, 2014-02-05;25(7):1151-8.
Species: Human
Sample Types: Cell Culture Supernates
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Gingival fibroblasts display reduced adhesion and spreading on extracellular matrix: a possible basis for scarless tissue repair?
Authors: Guo F, Carter DE, Mukhopadhyay A, Leask A
PLoS ONE, 2011-11-02;6(11):e27097.
Species: Human
Sample Types: Cell Culture Supernates
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Daily red wine consumption improves vascular function by a soluble guanylyl cyclase-dependent pathway.
Authors: Botden IP, Langendonk JG, Meima ME
Am. J. Hypertens., 2010-11-18;24(2):162-8.
Species: Human
Sample Types: Plasma
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Early life stress downregulates endothelin receptor expression and enhances acute stress-mediated blood pressure responses in adult rats.
Authors: Loria AS, D'Angelo G, Pollock DM, Pollock JS
Am. J. Physiol. Regul. Integr. Comp. Physiol., 2010-04-21;299(1):R185-91.
Species: Rat
Sample Types: Plasma
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Endothelin receptors blockade blunts hypoxia-induced increase in PAP in humans.
Authors: Pham I, Wuerzner G, Richalet JP, Peyrard S, Azizi M
Eur. J. Clin. Invest., 2010-03-01;40(3):195-202.
Species: Human
Sample Types: Plasma
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Vehicular emissions induce vascular MMP-9 expression and activity associated with endothelin-1-mediated pathways.
Authors: Lund AK, Lucero J, Lucas S, Madden MC, McDonald JD, Seagrave JC, Knuckles TL, Campen MJ
Arterioscler. Thromb. Vasc. Biol., 2009-01-15;29(4):511-7.
Species: Human
Sample Types: Plasma
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Effects of nonoral estradiol-micronized progesterone or low-dose oral estradiol-drospirenone therapy on metabolic variables and markers of endothelial function in early postmenopause.
Authors: Casanova G, Radavelli S, Lhullier F, Spritzer PM
Fertil. Steril., 2008-08-15;92(2):605-12.
Species: Human
Sample Types: Plasma
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Endothelial function, endothelin-1, and fibrinogen in young women using the vaginal contraceptive ring.
Authors: Torgrimson BN, Meendering JR, Miller NP, Kaplan PF, Minson CT
Fertil. Steril., 2008-08-15;92(2):441-7.
Species: Human
Sample Types: Serum
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Regulation of angiogenic factors by HDM2 in renal cell carcinoma.
Authors: Carroll VA, Ashcroft M
Cancer Res., 2008-01-15;68(2):545-52.
Species: Human
Sample Types: Cell Culture Supernates
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Eosinophil-derived cationic proteins activate the synthesis of remodeling factors by airway epithelial cells.
Authors: Pegorier S, Wagner LA, Gleich GJ, Pretolani M
J. Immunol., 2006-10-01;177(7):4861-9.
Species: Human
Sample Types: Cell Culture Supernates
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Digital blood flow and plasma endothelin concentration in clinically endotoxemic horses.
Authors: Menzies-Gow NJ, Bailey SR, Stevens K, Katz L, Elliott J, Marr CM
Am. J. Vet. Res., 2005-04-01;66(4):630-6.
Species: Equine
Sample Types: Plasma
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Mechanisms of elevated plasma endothelin-1 in CHF: congestion increases pulmonary synthesis and secretion of endothelin-1.
Authors: von Lueder TG, Kjekshus H, Edvardsen T, ØIe E, Urheim S, Vinge LE, Ahmed MS, Smiseth OA, Attramadal H
Cardiovasc. Res., 2004-07-01;63(1):41-50.
Species: Porcine
Sample Types: Plasma
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Novel endothelin receptor antagonist attenuates endotoxin-induced lung injury in sheep.
Authors: Kuklin VN, Kirov MY, Evgenov OV, Sovershaev MA, Sjoberg J, Kirova SS, Bjertnaes LJ
Crit. Care Med., 2004-03-01;32(3):766-73.
Species: Ovine
Sample Types: Plasma
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Renal endothelin in chronic angiotensin II hypertension.
Authors: Sasser JM, Pollock JS, Pollock DM
Am. J. Physiol. Regul. Integr. Comp. Physiol., 2002-07-01;283(1):R243-8.
Species: Rat
Sample Types: Tissue Homogenates
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Passive transfer of scrub typhus plasma to patients with AIDS: a descriptive clinical study.
Authors: Watt G, Kantipong P, Jongsakul K, de Souza M, Burnouf T
QJM, 2001-11-01;94(11):599-607.
Species: Human
Sample Types: Plasma
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Endothelin and ET(A) receptors in long-term arterial pressure homeostasis in conscious nonhuman primates.
Authors: Reinhart GA, 2018, Preusser LC, Opgenorth TJ, Wegner CD, Cox BF
1364165, 2000-11-01;279(5):R1701-6.
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Plasma
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undiluted human serum from healthy donors