Human Endothelin-3 Antibody

Catalog #: MAB5579 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5579
MAB5579-SP

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Human Endothelin-3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Endothelin‑3.

Source

Monoclonal Mouse IgG₁ Clone # 3B6

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human Endothelin-3 synthetic peptide
Accession # P14138

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Endothelin‑3 in LNCaP Human Cell Line. Endothelin-3 was detected in immersion fixed LNCaP human prostate cancer cell line using Mouse Anti-Human Endothelin-3 Monoclonal Antibody (Catalog # MAB5579) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endothelin-3

Endothelin-1, -2, and -3 are encoded by three separate genes as prepropolypeptides that are cleaved to yield the secreted and inactive big endothelins. Big
Endothelin-3 is further cleaved by Kell blood group antigen endopeptidases to generate the 21 amino acid (aa) bioactive mature peptide. It interacts primarily with the 7TM receptor EDNRB to induce a variety of effects including bronchoconstriction, arteriolar vasoconstriction, gall bladder contraction, insulin and norepinephrine release, and the maintenance and development of melanocytes and neural crest progenitors. Mature human Endothelin-3 shares 100% aa sequence identity with mouse and rat Endothelin-3.

Entrez Gene IDs

1908 (Human); 13616 (Mouse); 366270 (Rat)

Alternate Names

EDN3; endothelin 3; Endothelin3; Endothelin-3; ET3; ET-3; HSCR4; MGC15067; MGC61498; PPET3; preproendothelin-3; truncated endothelin 3; WS4B

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

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