Human ENPP-3/CD203c Fluorescein-conjugated Antibody

Name: Human ENPP-3/CD203c Fluorescein-conjugated Antibody
Brand: R&D Systems
SKU: FAB5756F

Catalog #: FAB5756F Datasheet / COA / SDS

Discontinued Product

FAB5756F has been discontinued.

View all ENPP-3/CD203c products.

Detection of ENPP‑3/CD203c in Human Whole Blood Lymphocytes and Monocytes by Flow Cytometry.

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All ENPP-3/CD203c Antibodies

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Human ENPP-3/CD203c Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ENPP‑3/CD203c.

Source

Monoclonal Mouse IgG₁ Clone # NP4D6

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human ENPP‑3/CD203c
Accession # O14638

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of ENPP‑3/CD203c in Human Whole Blood Lymphocytes and Monocytes by Flow Cytometry. Human whole blood lymphocytes and monocytes were stained with Mouse Anti-Human IL-3 Ra/CD123 PE-conjugated Monoclonal Antibody (Catalog # FAB301P) and either (A) Mouse Anti-Human ENPP-3/CD203c Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB5756F) or (B) Mouse IgG₁Fluorescein Isotype Control (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: ENPP-3/CD203c

CD203c is a 120-130 kDa type II transmembrane glycoprotein that belongs to the ectonucleotidase family, AP superfamily of enzymes. CD203c is also known as ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP-3) and it modulates purinergic signaling by hydrolysis of nucleotide di- and tri-phosphates plus sphingosylphosphorylenosine. Although CD203c is considered to be a marker for activated basophils and mast cells, it is also found constitutively or inducibly on bile duct epithelium, hepatocytes, duodenal columnar epithelium, osteoblasts, plasmacytoid dendritic cells, airway pseudostratified ciliated columnar epithelium and renal proximal tubule S3 segment cells. Within the ECD, human CD203c shares 81% aa sequence identity with mouse and rat CD203.

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 3

Entrez Gene IDs

5169 (Human)

Alternate Names

B10; CD203c antigen; CD203c; dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3); dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3); ectonucleotide pyrophosphatase/phosphodiesterase 3; ectonucleotide pyrophosphatase/phosphodiesterase family member 3; E-NPP 3; ENPP3; ENPP-3; gp130RB13-6; NPP3; PD-IBETA; PDNP3; PDNP3B10; Phosphodiesterase I beta; Phosphodiesterase I/nucleotide pyrophosphatase 3; phosphodiesterase-I beta