Human ENPP-5 Antibody

Catalog # Availability Size / Price Qty
MAB5979
MAB5979-SP
Product Details
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Human ENPP-5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ENPP-5 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) ENPP-2, rhENPP-7, or recombinant mouse ENPP-7 is observed.
Source
Monoclonal Mouse IgG2A Clone # 500915
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ENPP-5
Pro25-Ser430
Accession # Q9UJA9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human ENPP-5
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human ENPP-5, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ENPP-5

Ectonucleotide pyrophosphatase/phosphodiesterase 5 (ENPP-5) belongs to a group of nucleotide-metabolizing ecto-enzymes, which regulate the availability of extracellular nucleotides. It may also be involved in neuronal cell communication. The amino acid sequence of human ENPP-5 is 100%, 88%, and 82% identical to that of chimpanzee, dog and mouse/rat.

Long Name
Ectonucleotide Pyrophosphatase/Phosphodiesterase 5
Entrez Gene IDs
59084 (Human); 83965 (Mouse)
Alternate Names
EC 3.1; ectonucleotide pyrophosphatase/phosphodiesterase 5 (putative function); ectonucleotide pyrophosphatase/phosphodiesterase 5 (putative); ectonucleotide pyrophosphatase/phosphodiesterase family member 5; E-NPP 5; ENPP5; ENPP-5; KIAA0879; NPP5; NPP-5

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