Home / EOMES / Human EOMES Antibody

Human EOMES Antibody

Catalog #: AF6166
26 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6166
AF6166-SP
Detection of Human EOMES by Western Blot.
3 Images
Product Details
Citations (26)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EOMES Antibody Summary

Species Reactivity
Human
Specificity
Detects human EOMES in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human EOMES
Gly471-Pro686
Accession # O95936
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human EOMES antibody by Western Blot. View Larger

Detection of Human EOMES by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or mesoendoderm differentiated (+). PVDF Membrane was probed with 1 µg/mL of Human EOMES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6166) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for EOMES at approximately 85-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry EOMES antibody in mesoderm lineage cells differentiated from BG01V by Immunocytochemistry (ICC). View Larger

EOMES in mesoderm lineage cells differentiated from BG01V. EOMES was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Human EOMES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6166) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of EOMES antibody in Differ-entiated BG01V Human Cells antibody by Flow Cytometry. View Larger

Detection of EOMES in Differ-entiated BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells differentiated to mesendoderm were stained with Sheep Anti-Human EOMES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6166, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EOMES

EOMES (Eomesodermin; Eo from Greek meaning "dawn"/early in mesoderm; also TBR2) is a 72 kDa member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-gamma and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage. Human EOMES is 686 amino acids (aa) in length. It contains short poly-Ala, -Gly and -Asn motifs, and a DNA-binding T box (aa 276-456). There is one isoform that shows a 19 aa insertion after Ser460. Over aa 471‑686, human EOMES shares 91% aa identity with mouse EOMES.

Long Name
Eomesodermin Homolog
Entrez Gene IDs
8320 (Human); 13813 (Mouse); 316052 (Rat)
Alternate Names
EOMES; eomesodermin (Xenopus laevis) homolog; Eomesodermin; t box, brain, 2; T-box brain protein 2; T-box brain2; TBR2; TBR-2; TBR2eomesodermin homolog; T-brain-2

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human EOMES Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Human 3D cellular model of hypoxic brain injury of prematurity
    Authors: Wada H, Ikoma K, Oka Y et al.
    Nat. Med.
  2. Cerebral organoids display dynamic clonal growth and tunable tissue replenishment
    Authors: Lindenhofer, D;Haendeler, S;Esk, C;Littleboy, JB;Brunet Avalos, C;Naas, J;Pflug, FG;van de Ven, EGP;Reumann, D;Baffet, AD;von Haeseler, A;Knoblich, JA;
    Nature cell biology
    Species: Human
    Sample Types: Organoid
    Applications: Immunohistochemistry
  3. Joint epigenome profiling reveals cell-type-specific gene regulatory programmes in human cortical organoids
    Authors: Noack, F;Vangelisti, S;Ditzer, N;Chong, F;Albert, M;Bonev, B;
    Nature cell biology
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  4. Tissue morphology influences the temporal program of human brain organoid development
    Authors: Chiaradia, I;Imaz-Rosshandler, I;Nilges, BS;Boulanger, J;Pellegrini, L;Das, R;Kashikar, ND;Lancaster, MA;
    Cell stem cell
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  5. Single cell analysis of dup15q syndrome reveals developmental and postnatal molecular changes in autism
    Authors: Perez, Y;Velmeshev, D;Wang, L;White, M;Siebert, C;Baltazar, J;Dutton, NG;Wang, S;Haeussler, M;Chamberlain, S;Kriegstein, A;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Integrated transcriptome and proteome analysis reveals posttranscriptional regulation of ribosomal genes in human brain organoids
    Authors: J Sidhaye, P Trepte, N Sepke, M Novatchkov, M Schutzbier, G Dürnberger, K Mechtler, JA Knoblich
    Elife, 2023-03-29;12(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Single-cell RNA sequencing identifies a population of human liver-type ILC1s
    Authors: B Krämer, AP Nalin, F Ma, S Eickhoff, P Lutz, S Leonardell, F Goeser, C Finnemann, G Hack, J Raabe, M ToVinh, S Ahmad, C Hoffmeiste, KM Kaiser, S Manekeller, V Branchi, T Bald, M Hölzel, R Hüneburg, HD Nischalke, A Semaan, B Langhans, DJ Kaczmarek, B Benner, MR Lordo, J Kowalski, A Gerhardt, J Timm, M Toma, R Mohr, A Türler, A Charpentie, T van Bremen, G Feldmann, A Sattler, K Kotsch, AT Abdallah, CP Strassburg, U Spengler, WE Carson, BL Mundy-Boss, M Pellegrini, TE O'Sullivan, AG Freud, J Nattermann
    Cell Reports, 2023-01-01;42(1):111937.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  8. SARS-CoV-2 infects human brain organoids causing cell death and loss of synapses that can be rescued by treatment with Sofosbuvir
    Authors: P Mesci, JS de Souza, L Martin-San, A Macia, A Saleh, X Yin, C Snethlage, JW Adams, SH Avansini, RH Herai, A Almenar-Qu, Y Pu, RA Szeto, G Goldberg, PT Bruck, F Papes, SK Chanda, AR Muotri
    PloS Biology, 2022-11-03;20(11):e3001845.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  9. Androgens increase excitatory neurogenic potential in human brain organoids
    Authors: I Kelava, I Chiaradia, L Pellegrini, AT Kalinka, MA Lancaster
    Nature, 2022-01-19;0(0):.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  10. Endosomal trafficking defects alter neural progenitor proliferation and cause microcephaly
    Authors: Jacopo A. Carpentieri, Amandine Di Cicco, Marusa Lampic, David Andreau, Laurence Del Maestro, Fatima El Marjou et al.
    Nature Communications
  11. Developmental Origins of Human Cortical Oligodendrocytes and Astrocytes
    Authors: Lin Yang, Zhenmeiyu Li, Guoping Liu, Xiaosu Li, Zhengang Yang
    Neuroscience Bulletin
  12. Human microglia states are conserved across experimental models and regulate neural stem cell responses in chimeric organoids
    Authors: G Popova, SS Soliman, CN Kim, MG Keefe, KM Hennick, S Jain, T Li, D Tejera, D Shin, BB Chhun, CS McGinnis, M Speir, ZJ Gartner, SB Mehta, M Haeussler, KB Hengen, RR Ransohoff, X Piao, TJ Nowakowski
    Cell Stem Cell, 2021-09-17;0(0):.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  13. The CTNNBIP1-CLSTN1 fusion transcript regulates human neocortical development
    Authors: MY Ou, Q Xiao, XC Ju, PM Zeng, J Huang, AL Sheng, ZG Luo
    Cell Reports, 2021-06-29;35(13):109290.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  14. Developmental Differences in Neocortex Neurogenesis and Maturation Between the Altricial Dwarf Rabbit and Precocial Guinea Pig
    Authors: Mirjam Kalusa, Maren D. Heinrich, Christine Sauerland, Markus Morawski, Simone A. Fietz
    Frontiers in Neuroanatomy
  15. An early cell shape transition drives evolutionary expansion of the human forebrain
    Authors: Silvia Benito-Kwiecinski, Stefano L. Giandomenico, Magdalena Sutcliffe, Erlend S. Riis, Paula Freire-Pritchett, Iva Kelava et al.
    Cell
  16. Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia
    Authors: Ugomma C. Eze, Aparna Bhaduri, Maximilian Haeussler, Tomasz J. Nowakowski, Arnold R. Kriegstein
    Nature Neuroscience
  17. SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids
    Authors: L Pellegrini, A Albecka, DL Mallery, MJ Kellner, D Paul, AP Carter, LC James, MA Lancaster
    Cell Stem Cell, 2020-10-13;0(0):.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  18. mTOR signaling regulates the morphology and migration of outer radial glia in developing human cortex
    Authors: Madeline G Andrews, Lakshmi Subramanian, Arnold R Kriegstein
    eLife
  19. Origins and Proliferative States of Human Oligodendrocyte Precursor Cells
    Authors: Wei Huang, Aparna Bhaduri, Dmitry Velmeshev, Shaohui Wang, Li Wang, Catherine A. Rottkamp et al.
    Cell
  20. A discrete subtype of neural progenitor crucial for cortical folding in the gyrencephalic mammalian brain
    Authors: N Matsumoto, S Tanaka, T Horiike, Y Shinmyo, H Kawasaki
    Elife, 2020-04-21;9(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Extracellular matrix-inducing Sox9 promotes both basal progenitor proliferation and gliogenesis in developing neocortex
    Authors: Ayse Güven, Nereo Kalebic, Katherine R Long, Marta Florio, Samir Vaid, Holger Brandl et al.
    eLife
  22. Signs of Reduced Basal Progenitor Levels and Cortical Neurogenesis in Human Fetuses with Open Spina Bifida at 11–15 Weeks of Gestation
    Authors: Simone A. Fietz, Takashi Namba, Holger Kirsten, Wieland B. Huttner, Robert Lachmann
    The Journal of Neuroscience
  23. Microglia: An Intrinsic Component of the Proliferative Zones in the Fetal Rhesus Monkey (Macaca mulatta) Cerebral Cortex
    Authors: Nicole Barger, Janet Keiter, Anna Kreutz, Anjana Krishnamurthy, Cody Weidenthaler, Verónica Martínez-Cerdeño et al.
    Cerebral Cortex
  24. Reprogramming to pluripotency does not require transition through a primitive streak-like state
    Authors: S Raab, M Klingenste, A Möller, A Illing, J Tosic, M Breunig, G Kuales, L Linta, T Seufferlei, SJ Arnold, A Kleger, S Liebau
    Sci Rep, 2017-11-29;7(1):16543.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  25. Differences and similarities between human and chimpanzee neural progenitors during cerebral cortex development
    Authors: Felipe Mora-Bermúdez, Farhath Badsha, Sabina Kanton, J Gray Camp, Benjamin Vernot, Kathrin Köhler et al.
    eLife
  26. Establishing Cerebral Organoids as Models of Human-Specific Brain Evolution
    Authors: Pollen AA, Bhaduri A, Andrews MG et al.
    Cell

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human EOMES Antibody

There are currently no reviews for this product. Be the first to review Human EOMES Antibody and earn rewards!

Have you used Human EOMES Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review