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Human Ephrin-A5 Antibody

Catalog #: AF3743
14 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3743
AF3743-SP
Ephrin‑A5 in Human Melanoma.
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Product Details
Citations (14)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Ephrin-A5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Ephrin-A5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Ephrin-A1, rmEphrin-A2, recombinant human (rh) Ephrin-A3, rhEphrin-A4, rhEphrin-B3, rmEphrin-A4, rmEphrin-B1 and rmEphrin-B2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Ephrin-A5
Gln21-Asn203
Accession # P52803
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Ephrin-A5 Fc Chimera (Catalog # 374-EA)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Ephrin-A5 antibody in Human Melanoma by Immunohistochemistry (IHC-P). View Larger

Ephrin‑A5 in Human Melanoma. Ephrin-A5 was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-Human Ephrin-A5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3743) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-A5

Ephrin-A5, also known as AL-1, RAGS, LERK-7, and EFL-5 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A5 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphA8 (2, 3). The extracellular domains of murine and human Ephrin-A5 share 99% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The ephrin/Eph families also appear to play a role in angiogenesis (3).

References
  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
Entrez Gene IDs
1946 (Human); 13640 (Mouse)
Alternate Names
AF1; AL-1; EFL-5; EFNA5; EPH-related receptor tyrosine kinase ligand 7; EphrinA5; Ephrin-A5; EPLG7; GLC1M; LERK-7; LERK7EFL5; RAGS

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Citations for Human Ephrin-A5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Mutant alpha 2-chimaerin signals via bidirectional ephrin pathways in Duane retraction syndrome
    Authors: Alicia A. Nugent, Jong G. Park, Yan Wei, Alan P. Tenney, Nicole M. Gilette, Michelle M. DeLisle et al.
    Journal of Clinical Investigation
  2. Notch-mediated Ephrin signaling disrupts islet architecture and beta cell function
    Authors: A Bartolomé, N Suda, J Yu, C Zhu, J Son, H Ding, A Califano, D Accili, UB Pajvani
    JCI Insight, 2022-03-22;0(0):.
  3. ADAM10-cleaved ephrin-A5 contributes to prostate cancer metastasis
    Authors: C Cai, M Zhang, L Liu, H Zhang, Y Guo, T Lan, Y Xu, P Ma, S Li
    Cell Death & Disease, 2022-05-12;13(5):453.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Immunoprecipitation
  4. The Value of EphB2 Receptor and Cognate Ephrin Ligands in Prognostic and Predictive Assessments of Human Breast Cancer
    Authors: Abdul Shukkur Ebrahim, Zeyad Hailat, Sudeshna Bandyopadhyay, Daniel Neill, Mustapha Kandouz
    International Journal of Molecular Sciences
  5. EphA4 may contribute to microvessel remodeling in the hippocampal CA1 and CA3 areas in a mouse model of temporal lobe epilepsy
    Mol Med Rep, 2016-12-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Role of synaptic plasticity and EphA5-ephrinA5 interaction within the ventromedial hypothalamus in response to recurrent hypoglycemia.
    Authors: Szepietowska, Barbara, Horvath, Tamas L, Sherwin, Robert S
    Diabetes, 2013-11-12;63(3):1140-7.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. EphA5-EphrinA5 interactions within the ventromedial hypothalamus influence counterregulatory hormone release and local glutamine/glutamate balance during hypoglycemia.
    Authors: Szepietowska B, Zhu W, Czyzyk J, Eid T, Sherwin R
    Diabetes, 2012-12-28;62(4):1282-8.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. beta3GnT2 null mice exhibit defective accessory olfactory bulb innervation.
    Authors: Henion T, Madany P, Faden A, Schwarting G
    Mol Cell Neurosci, 2012-09-21;52(0):73-86.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  9. EphrinA5 acts as a tumor suppressor in glioma by negative regulation of epidermal growth factor receptor.
    Authors: Li JJ, Liu DP, Liu GT, Xie D
    Oncogene, 2009-03-09;28(15):1759-68.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  10. Meltrin beta/ADAM19 interacting with EphA4 in developing neural cells participates in formation of the neuromuscular junction.
    Authors: Yumoto N, Wakatsuki S, Kurisaki T, Hara Y, Osumi N, Frisen J, Sehara-Fujisawa A
    PLoS ONE, 2008-10-02;3(10):e3322.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. p75(NTR) mediates ephrin-A reverse signaling required for axon repulsion and mapping.
    Authors: Lim YS, McLaughlin T, Sung TC, Santiago A, Lee KF, O'Leary DD
    Neuron, 2008-09-11;59(5):746-58.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. BIG-2 mediates olfactory axon convergence to target glomeruli.
    Authors: Kaneko-Goto T, Yoshihara S, Miyazaki H, Yoshihara Y
    Neuron, 2008-03-27;57(6):834-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. EphA-Ephrin-A-mediated beta cell communication regulates insulin secretion from pancreatic islets.
    Authors: Konstantinova I, Nikolova G, Ohara-Imaizumi M, Meda P, Kucera T, Zarbalis K, Wurst W, Nagamatsu S, Lammert E
    Cell, 2007-04-20;129(2):359-70.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Adam meets Eph: an ADAM substrate recognition module acts as a molecular switch for ephrin cleavage in trans.
    Authors: Janes PW, Saha N, Barton WA, Kolev MV, Wimmer-Kleikamp SH, Nievergall E, Blobel CP, Himanen JP, Lackmann M, Nikolov DB
    Cell, 2005-10-21;123(2):291-304.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot

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Human Ephrin-A5 Antibody
By Anonymous on 02/18/2019
Application: WB Sample Tested: DA3 mouse myeloma cell line Species: Mouse
Western Blot Human Ephrin-A5 Antibody AF3743
Human Ephrin-A5 Antibody
By Anonymous on 10/17/2018
Application: WB Sample Tested: AR42J human acinar pancreatic cancer cell line,Capan-1 human pancreatic adenocarcinoma cell line Species: Human
Western Blot Human Ephrin-A5 Antibody AF3743