Human ERK3 Antibody

Catalog #: AF3196 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3196
AF3196-SP

Detection of Human ERK3 by Western Blot.

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Human ERK3 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human ERK3 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ERK3
Met344-Asn721
Accession # Q16659

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ERK3 by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line, U937 human histiocytic lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human ERK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3196) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ERK3 at approximately 105 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERK3

Extracellular signal-regulated kinase 3 (ERK3) is a serine/threonine protein kinase also known as mitogen-activated protein kinase 6 (MAPK6) and p97MAPK. Unlike ERK1 and ERK2, ERK3 lacks the phosphoacceptor sequence T-E-Y in its activation loop. This kinase also possesses a unique C-terminal extension that is missing in traditional MAPKs. Highly unstable, ERK3 is rapidly degraded following N-terminal ubiquitination.

Long Name

Extracellular Signal-regulated Kinase 3

Entrez Gene IDs

5597 (Human); 50772 (Mouse); 58840 (Rat)

Alternate Names

DKFZp686F03189; EC 2.7.11; EC 2.7.11.24; ERK3; ERK-3; ERK3p97-MAPK; Extracellular signal-regulated kinase 3; extracellular signal-regulated kinase, p97; HsT17250; MAP kinase 6; MAP kinase isoform p97; MAPK 6; MAPK6; mitogen-activated protein kinase 6; p97MAPK; PRKM6; protein kinase, mitogen-activated 5; protein kinase, mitogen-activated 6