Human Erythropoietin R Antibody

Catalog #: MAB3072 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3072
MAB3072-SP

Detection of Human Erythropoietin R by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Erythropoietin R Antibody Summary

Species Reactivity

Human

Specificity

Detects human Erythropoietin R in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 690748

Purification

Protein A or G purified from ascites

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Y426 of human Erythropoietin R
Accession # P19235

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

KU812 Human Chronic Myelogenous Leukemic cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Erythropoietin R by Western Blot. Western blot shows lysates of UT-7 human acute myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB3072) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). Specific bands were detected for Erythropoietin R at approximately 64 to 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of Erythropoietin R in KU812 cells by Flow Cytometry KU812 cells were stained with Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB3072, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Erythropoietin R

Erythropoietin (Epo), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The biological effects of Epo are mediated by the Erythropoietin Receptor (Epo R). The full length human Epo R cDNA encodes a type I membrane-spanning protein with 508 amino acid (aa) (a 24 aa hydrophobic signal sequence, a 226 aa extracellular domain, a 22 aa transmembrane domain and a 236 aa cytoplasmic domain). At the protein sequence level, the human Epo R is approximately 82% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding a truncated form of the Epo R as well as the soluble form of Epo R has been found. The presence of a soluble form of the Epo R has also been detected in human sera. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.

References

Barber, D.L. and A.D. D'Andrea (1992) Seminars in Hematology 29:293.
Youssoufian, H. et al. (1993) Blood 9:2223.
Lodish, H.F. et al. (1995) Cold Spring Harbor Symposia on Quantitative Biology LX:93.
Baynes, R.D. et al. (1993) Blood 82:2088.

Long Name

Erythropoietin Receptor

Entrez Gene IDs

2057 (Human); 13857 (Mouse)

Alternate Names

EpoR; EPO-R; Erythropoietin R; erythropoietin receptor; MGC138358