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Human Erythropoietin R Antibody

Catalog #: AF-322-PB
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-322-PB
AF-322-SP
Detection of Human Erythropoietin R by Western Blot.
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Product Details
Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Erythropoietin R Antibody Summary

Species Reactivity
Human
Specificity
Detects human Erythropoietin R in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with rhEpo and rhTpo is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Erythropoietin R
Pro26-Pro250
Accession # P19235
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Erythropoietin R antibody by Western Blot. View Larger

Detection of Human Erythropoietin R by Western Blot. Western blot shows lysates of UT-7 human acute myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Erythropoietin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-322-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Erythropoietin R at approximately 70-78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Erythropoietin R antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Erythropoietin R by Simple WesternTM. Simple Western lane view shows lysates of UT-7 human acute myeloid leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Erythropoietin R at approximately 76 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Erythropoietin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-322-PB) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Erythropoietin R

Erythropoietin (Epo), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The biological effects of Epo are mediated by the erythropoietin receptor (Epo R). The genes for human and mouse Epo R have been cloned and characterized. The full-length human Epo R cDNA encodes a type I membrane-spanning protein with 508 amino acid (aa) residues (a 24 aa residue hydrophobic signal sequence, a 226 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 236 aa residue cytoplasmic domain). At the protein sequence level, the human Epo R is approximately 82% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding a truncated form of the Epo R as well as the soluble form of Epo R has been found. The presence of a soluble form of the Epo R has also been detected on human sera. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.

References
  1. Barber, D.L. and A.D. D’Andrea (1992) Seminars in Hematology 29:293.
  2. Youssoufian, H. et al. (1993) Blood 9:2223.
  3. Lodish, H.F. et al. (1995) Cold Spring Harbor Symposia on Quantitative Biology LX:93.
  4. Baynes, R.D. et al. (1993) Blood 82:2088.
Long Name
Erythropoietin Receptor
Entrez Gene IDs
2057 (Human); 13857 (Mouse)
Alternate Names
EpoR; EPO-R; Erythropoietin R; erythropoietin receptor; MGC138358

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Citations for Human Erythropoietin R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Far-western blotting as a solution to the non-specificity of the anti-erythropoietin receptor antibody
    Authors: Barbora Fecková, Patrícia Kimáková, Lenka Ilkovičová, Erika Szentpéteriová, Nataša Debeljak, Zuzana Solárová et al.
    Oncology Letters
  2. Location and the functionality of erythropoietin receptor(s) in A2780 cells
    Authors: Peter Solár, Gabriela Hrčková, Lenka Varinská, Zuzana Solárová, Ján Kriška, Ivana Uhrínová et al.
    Oncology Reports
  3. Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
    Authors: Khankin EV, Mutter WP, Tamez H
    PLoS ONE, 2010-02-16;5(2):e9246.
    Species: Human
    Sample Types: Serum
    Applications: Immunoprecipitation, Western Blot
  4. Functional and immunochemical characterisation of different antibodies against the erythropoietin receptor.
    Authors: Kirkeby A, van Beek J, Nielsen J, Leist M, Helboe L
    J Neurosci Methods, 2007-04-12;164(1):50-8.
    Species: Human, Rat
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  5. Methylation of the first exon in the erythropoietin receptor gene does not correlate with its mRNA and protein level in cancer cells
    Authors: B Fecková, P Kimáková, L Ilkovi?ová, E Szentpéter, M Macejová, J Košuth, A Zulli, N Debeljak, P Hudler, K Jašek, I Kašubová, P Kubatka, P Solár
    BMC Genet., 2019-01-03;20(1):1.

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