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Human Erythropoietin R APC-conjugated Antibody

Catalog #: FAB3072A
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB3072A
Detection of Erythropoietin R in TF‑1 Human Cell Line by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Erythropoietin R APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Epo R in ELISAs. In ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) Epo, rhTpo R, or rmEpo R.
Source
Monoclonal Mouse IgG1 Clone # 38421
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Erythropoietin R
Ala25-Pro250
Accession # P19235
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Erythropoietin R antibody in TF-1 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Erythropoietin R in TF‑1 Human Cell Line by Flow Cytometry. TF-1 human erythroleukemic cell line was stained with Mouse Anti-Human Erythropoietin R APC-conjugated Monoclonal Antibody (Catalog # FAB3072A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Erythropoietin R

Epo R is a transmembrane protein expressed on the surface of megakaryocytes, erythroid progenitors, and endothelial cells. It binds Epo and transmits signals that stimulate the proliferation and maturation of bone marrow erythroid precursors into red cells.

Long Name
Erythropoietin Receptor
Entrez Gene IDs
2057 (Human); 13857 (Mouse)
Alternate Names
EpoR; EPO-R; Erythropoietin R; erythropoietin receptor; MGC138358

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Citations for Human Erythropoietin R APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Nicotinamide mononucleotide (NMN) supplementation rescues cerebromicrovascular endothelial function and neurovascular coupling responses and improves cognitive function in aged mice
    Authors: S Tarantini, MN Valcarcel-, P Toth, A Yabluchans, Z Tucsek, T Kiss, P Hertelendy, M Kinter, P Ballabh, Z Süle, E Farkas, JA Baur, DA Sinclair, A Csiszar, Z Ungvari
    Redox Biol, 2019-04-10;24(0):101192.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Truncating Erythropoietin Receptor Rearrangements in Acute Lymphoblastic Leukemia
    Authors: Ilaria Iacobucci, Yongjin Li, Kathryn G. Roberts, Stephanie M. Dobson, Jaeseung C. Kim, Debbie Payne-Turner et al.
    Cancer Cell

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