Human Erythropoietin R Biotinylated Antibody

Catalog #: BAM3072 Datasheet / COA / SDS

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BAM3072

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Erythropoietin R Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Epo R in ELISAs. In ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) Epo, rhTpo R, or rmEpo R.

Source

Monoclonal Mouse IgG₁ Clone # 38421

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Erythropoietin R
Ala25-Pro250
Accession # P19235

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Human Erythropoietin R Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Human Erythropoietin R Antibody (Catalog # MAB3071)

Standard: Recombinant Human Erythropoietin R Protein (Catalog # 307-ER)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Erythropoietin R

Epo R is a transmembrane protein expressed on the surface of megakaryocytes, erythroid progenitors, and endothelial cells. It binds Epo and transmits signals that stimulate the proliferation and maturation of bone marrow erythroid precursors into red cells.

Long Name

Erythropoietin Receptor

Entrez Gene IDs

2057 (Human); 13857 (Mouse)

Alternate Names

EpoR; EPO-R; Erythropoietin R; erythropoietin receptor; MGC138358