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Human Erythropoietin R Fluorescein-conjugated Antibody

Catalog #: FAB307F
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB307F
Detection of Erythropoietin R in TF‑1 Human Cell Line by Flow Cytometry.
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Product Details
Citations (4)
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Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Erythropoietin R Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Erythropoietin R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Erythropoietin R is observed.
Source
Monoclonal Mouse IgG2B Clone # 38409
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Erythropoietin R
Pro26-Pro250
Accession # P19235
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
TF‑1 human erythroleukemic cell line; KU812 human chronic myelogenous leukemic cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Erythropoietin R antibody in TF‑1 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Erythropoietin R in TF‑1 Human Cell Line by Flow Cytometry. TF-1 human erythroleukemic cell line was stained with Mouse Anti-Human Erythropoietin R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB307F, filled histogram) or isotype control antibody (IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of Erythropoietin R in KU812 cells by Flow Cytometry KU812 cells were stained with Mouse Anti-Human Erythropoietin R Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB307F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Erythropoietin R

Erythropoietin R is a transmembrane protein expressed on the surface of megakaryocytes, erythroid progenitors, and endothelial cells. It binds Erythropoietin and transmits signals that stimulate the proliferation and maturation of bone marrow erythroid precursors into red cells.

Long Name
Erythropoietin Receptor
Entrez Gene IDs
2057 (Human); 13857 (Mouse)
Alternate Names
EpoR; EPO-R; Erythropoietin R; erythropoietin receptor; MGC138358

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Citations for Human Erythropoietin R Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Plant-Produced Human Recombinant Erythropoietic Growth Factors Support Erythroid Differentiation In Vitro
    Authors: Konstantin Musiychuk, Rajarajeswari Sivalenka, Jennifer Jaje, Hong Bi, Rosemary Flores, Brenden Shaw et al.
    Stem Cells and Development
  2. Location and the functionality of erythropoietin receptor(s) in A2780 cells
    Authors: Peter Solár, Gabriela Hrčková, Lenka Varinská, Zuzana Solárová, Ján Kriška, Ivana Uhrínová et al.
    Oncology Reports
  3. Erythropoietin mimetic compound AGEM400(HES) binds to the same receptor as erythropoietin but displays a different spectrum of activities.
    Authors: Kessler C, Greindl A, Breuer B, Haberl U, Rybka A, Emgenbroich M, Frank HG, Potgens AJ
    Cytokine, 2011-12-03;57(2):226-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Hypoxia-inducible factors in human pulmonary arterial hypertension: a link to the intrinsic myeloid abnormalities.
    Authors: Farha S, Asosingh K, Xu W, Sharp J, George D, Comhair S, Park M, Tang WH, Loyd JE, Theil K, Tubbs R, Hsi E, Lichtin A, Erzurum SC
    Blood, 2011-01-21;117(13):3485-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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