Human Erythropoietin R MAb (Clone 713210)

Catalog #: MAB3073 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3073-100
MAB3073-SP

Proliferation Induced by Erythropoietin and Neutralization by Human Erythropoietin R Antibody.

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Human Erythropoietin R MAb (Clone 713210) Summary

Species Reactivity

Human

Specificity

Detects human Erythropoietin R in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 713210

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Erythropoietin R
Ala25-Pro250
Accession # P19235

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize Erythropoietin-induced proliferation in the TF‑1 human erythroleukemic cell line. The Neutralization Dose (ND₅₀) is typically 0.4-2.4 µg/mL in the presence of 0.278 units/mL Recombinant Human Erythropoietin (Tissue Culture Grade).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Proliferation Induced by Erythropoietin and Neutralization by Human Erythropoietin R Antibody. Recombinant Human Erythropoietin (Tissue Culture Grade) (Catalog # 287-TC) induces proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Erythropoietin (Tissue Culture Grade) (0.278 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB3073). The ND₅₀ is typically 0.4-2.4 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Erythropoietin R

Epo R is a transmembrane protein expressed on the surface of megakaryocytes, erythroid progenitors, and endothelial cells. It binds Epo and transmits signals that stimulate the proliferation and maturation of bone marrow erythroid precursors into red cells.

Long Name

Erythropoietin Receptor

Entrez Gene IDs

2057 (Human); 13857 (Mouse)

Alternate Names

EpoR; EPO-R; Erythropoietin R; erythropoietin receptor; MGC138358